Holmes et al. 2023 (PRJNA1017399)
General Details
| Title | Depletion of mRNA cap-binding protein eIF4E1 drives bradyzoite formation in Toxoplasma gondii [RIBO-seq] |
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| Organism | |
| Number of Samples | 6 |
| Release Date | 2023/09/14 00:00 |
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| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP460544 |
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| ENA | SRP460544 |
| GEO | |
| BioProject | PRJNA1017399 |
Publication
| Title | |
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| Authors | Holmes MJ,Bastos MS,Dey V,Severo V,Wek RC,Sullivan WJ Jr |
| Journal | bioRxiv : the preprint server for biology |
| Publication Date | 2023 Oct 9 |
| Abstract | The protozoan parasite Toxoplasma gondii causes serious opportunistic disease due to its ability to persist in patients as latent tissue cysts. The molecular mechanisms coordinating conversion between proliferative parasites (tachyzoites) and dormant cysts (bradyzoites) are not fully understood. We previously showed that phosphorylation of eIF2α accompanies bradyzoite formation, suggesting that this clinically relevant process involves regulation of mRNA translation. In this study, we investigated the composition and role of eIF4F multi-subunit complexes in translational control. Using CLIPseq, we find that the cap-binding subunit, eIF4E1, localizes to the 5'-end of all tachyzoite mRNAs, many of which show evidence of stemming from heterogenous transcriptional start sites. We further show that eIF4E1 operates as the predominant cap-binding protein in two distinct eIF4F complexes. Using genetic and pharmacological approaches, we found that eIF4E1 deficiency triggers efficient spontaneous formation of bradyzoites without stress induction. Consistent with this result, we also show that stress-induced bradyzoites exhibit reduced eIF4E1 expression. Overall, our findings establish a novel role for eIF4F in translational control required for parasite latency and microbial persistence. |
| PMC | PMC10592687 |
| PMID | 37873335 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR26060428 | PRJNA1017399 | Toxoplasma gondii | RH::TIR1 parental straintachyzoites |  |
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| SRR26060427 | PRJNA1017399 | Toxoplasma gondii | RH::TIR1 parental straintachyzoites | |
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| SRR26060426 | PRJNA1017399 | Toxoplasma gondii | RH::TIR1 parental straintachyzoites | |
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| SRR26060425 | PRJNA1017399 | Toxoplasma gondii | RH::TIR1 parental straintachyzoites | |
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| SRR26060424 | PRJNA1017399 | Toxoplasma gondii | RH::TIR1 parental straintachyzoites | |
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| SRR26060423 | PRJNA1017399 | Toxoplasma gondii | RH::TIR1 parental straintachyzoites | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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