Bazzini et al. 2012 (PRJNA150397)

General Details

Title Ribosome profiling of early zebrafish embryos -- miRNA-mediated regulation during embryogenesis causes translational repression before mRNA decay
Organism
Number of Samples 34
Release Date 2012/03/15 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP010040
ENA SRP010040
GEO GSE34743
BioProject PRJNA150397

Publication

Title
Authors Bazzini AA, Lee MT, Giraldez AJ
Journal Science (New York, N.Y.)
Publication Date 2012 Apr 13
Abstract MicroRNAs regulate gene expression through deadenylation, repression, and messenger RNA (mRNA) decay. However, the contribution of each mechanism in non-steady-state situations remains unclear. We monitored the impact of miR-430 on ribosome occupancy of endogenous mRNAs in wild-type and dicer mutant zebrafish embryos and found that miR-430 reduces the number of ribosomes on target mRNAs before causing mRNA decay. Translational repression occurs before complete deadenylation, and disrupting deadenylation with use of an internal polyadenylate tail did not block target repression. Lastly, we observed that ribosome density along the length of the message remains constant, suggesting that translational repression occurs by reducing the rate of initiation rather than affecting elongation or causing ribosomal drop-off. These results show that miR-430 regulates translation initiation before inducing mRNA decay during zebrafish development.
PMC PMC3547538
PMID 22422859
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR392976 PRJNA150397 Danio rerio 0.0 RNA-Seq 0.0
SRR392977 PRJNA150397 Danio rerio 0.0 RNA-Seq 0.0
SRR392978 PRJNA150397 Danio rerio 0.0 RNA-Seq 0.0
SRR392979 PRJNA150397 Danio rerio 0.0 RNA-Seq 0.0
SRR392980 PRJNA150397 Danio rerio 0.0 RNA-Seq 0.0
SRR392981 PRJNA150397 Danio rerio 0.0 RNA-Seq 0.0
SRR392982 PRJNA150397 Danio rerio 0.0 RNA-Seq 0.0
SRR392983 PRJNA150397 Danio rerio 0.0 RNA-Seq 0.0
SRR392984 PRJNA150397 Danio rerio 0.0 RNA-Seq 0.0
SRR392985 PRJNA150397 Danio rerio 0.0 RNA-Seq 0.0
SRR392986 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392987 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392988 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392989 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392990 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392991 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392992 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392993 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392994 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392995 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392996 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392997 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392998 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR392999 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR393000 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR393001 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR393002 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR393003 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR393004 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR393005 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR393006 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR393007 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR393008 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
SRR393009 PRJNA150397 Danio rerio 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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