Chew et al. 2013 (PRJNA200706)
General Details
| Title | Ribosome Profiling over a Zebrafish Developmental Timecourse |
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| Organism | |
| Number of Samples | 8 |
| Release Date | 2013/04/30 00:00 |
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| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP021915 |
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| ENA | SRP021915 |
| GEO | GSE46512 |
| BioProject | PRJNA200706 |
Publication
| Title | |
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| Authors | Chew GL, Pauli A, Rinn JL, Regev A, Schier AF, Valen E |
| Journal | Development (Cambridge, England) |
| Publication Date | 2013 Jul |
| Abstract | Large-scale genomics and computational approaches have identified thousands of putative long non-coding RNAs (lncRNAs). It has been controversial, however, as to what fraction of these RNAs is truly non-coding. Here, we combine ribosome profiling with a machine-learning approach to validate lncRNAs during zebrafish development in a high throughput manner. We find that dozens of proposed lncRNAs are protein-coding contaminants and that many lncRNAs have ribosome profiles that resemble the 5' leaders of coding RNAs. Analysis of ribosome profiling data from embryonic stem cells reveals similar properties for mammalian lncRNAs. These results clarify the annotation of developmental lncRNAs and suggest a potential role for translation in lncRNA regulation. In addition, our computational pipeline and ribosome profiling data provide a powerful resource for the identification of translated open reading frames during zebrafish development. |
| PMC | PMC3678345 |
| PMID | 23698349 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR836192 | PRJNA200706 | Danio rerio | 0.0 | Ribo-Seq | Cycloheximide |  |
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| SRR836193 | PRJNA200706 | Danio rerio | 0.0 | Ribo-Seq | Cycloheximide | |
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| SRR836194 | PRJNA200706 | Danio rerio | 0.0 | Ribo-Seq | Cycloheximide | |
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| SRR836195 | PRJNA200706 | Danio rerio | 0.0 | Ribo-Seq | Cycloheximide | |
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| SRR836196 | PRJNA200706 | Danio rerio | 0.0 | Ribo-Seq | Cycloheximide | |
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| SRR836197 | PRJNA200706 | Danio rerio | 0.0 | Ribo-Seq | Cycloheximide | |
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| SRR836198 | PRJNA200706 | Danio rerio | 0.0 | Ribo-Seq | Cycloheximide | |
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| SRR836199 | PRJNA200706 | Danio rerio | 0.0 | Ribo-Seq | 0.0 | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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