Gilmore et al. 2015 (PRJNA283425)

General Details

Title Genome-wide reprogramming of transcript architecture by temperature specifies the developmental states of the human pathogen Histoplasma [ribosome profiling]
Organism
Number of Samples 4
Release Date 2015/05/08 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP058148
ENA SRP058148
GEO GSE68705
BioProject PRJNA283425

Publication

Title
Authors Gilmore SA, Voorhies M, Gebhart D, Sil A
Journal PLoS genetics
Publication Date 2015 Jul
Abstract Eukaryotic cells integrate layers of gene regulation to coordinate complex cellular processes; however, mechanisms of post-transcriptional gene regulation remain poorly studied. The human fungal pathogen Histoplasma capsulatum (Hc) responds to environmental or host temperature by initiating unique transcriptional programs to specify multicellular (hyphae) or unicellular (yeast) developmental states that function in infectivity or pathogenesis, respectively. Here we used recent advances in next-generation sequencing to uncover a novel re-programming of transcript length between Hc developmental cell types. We found that ~2% percent of Hc transcripts exhibit 5' leader sequences that differ markedly in length between morphogenetic states. Ribosome density and mRNA abundance measurements of differential leader transcripts revealed nuanced transcriptional and translational regulation. One such class of regulated longer leader transcripts exhibited tight transcriptional and translational repression. Further examination of these dually repressed genes revealed that some control Hc morphology and that their strict regulation is necessary for the pathogen to make appropriate developmental decisions in response to temperature.
PMC PMC4503680
PMID 26177267
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR2015208 PRJNA283425 Histoplasma capsulatum 0.0 Ribo-Seq 0.0
SRR2015210 PRJNA283425 Histoplasma capsulatum 0.0 Ribo-Seq 0.0
SRR2015212 PRJNA283425 Histoplasma capsulatum 0.0 Ribo-Seq 0.0
SRR2015214 PRJNA283425 Histoplasma capsulatum 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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