Jeong et al. 2016 (PRJNA285265)

General Details

Title Transcriptome and translatome landscape of Streptomyces coelicolor M145
Organism
Number of Samples 4
Release Date 2015/05/28 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP058830
ENA SRP058830
GEO GSE69350
BioProject PRJNA285265

Publication

Title
Authors Jeong Y, Kim JN, Kim MW, Bucca G, Cho S, Yoon YJ, Kim BG, Roe JH, Kim SC, Smith CP, Cho BK
Journal Nature communications
Publication Date 2016 Jun 2
Abstract Individual Streptomyces species have the genetic potential to produce a diverse array of natural products of commercial, medical and veterinary interest. However, these products are often not detectable under laboratory culture conditions. To harness their full biosynthetic potential, it is important to develop a detailed understanding of the regulatory networks that orchestrate their metabolism. Here we integrate nucleotide resolution genome-scale measurements of the transcriptome and translatome of Streptomyces coelicolor, the model antibiotic-producing actinomycete. Our systematic study determines 3,570 transcription start sites and identifies 230 small RNAs and a considerable proportion (∼21%) of leaderless mRNAs; this enables deduction of genome-wide promoter architecture. Ribosome profiling reveals that the translation efficiency of secondary metabolic genes is negatively correlated with transcription and that several key antibiotic regulatory genes are translationally induced at transition growth phase. These findings might facilitate the design of new approaches to antibiotic discovery and development.
PMC PMC4895711
PMID 27251447
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR2043966 PRJNA285265 Streptomyces coelicolor 0.0 Ribo-Seq 0.0
SRR2043967 PRJNA285265 Streptomyces coelicolor 0.0 Ribo-Seq 0.0
SRR2043968 PRJNA285265 Streptomyces coelicolor 0.0 Ribo-Seq 0.0
SRR2043969 PRJNA285265 Streptomyces coelicolor 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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