Guydosh et al. 2017 (PRJNA386877)
General Details
Title | Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe |
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Organism | |
Number of Samples | 10 |
Release Date | 2017/05/15 00:00 |
Sequencing Types | |
Protocol Details |
Study Links
GWIPS-viz | Trips-Viz |
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Repository Details
SRA | SRP107240 |
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ENA | SRP107240 |
GEO | GSE98934 |
BioProject | PRJNA386877 |
Publication
Title | |
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Authors | Guydosh NR, Kimmig P, Walter P, Green R |
Journal | eLife |
Publication Date | 2017 Sep 25 |
Abstract | The unfolded protein response (UPR) monitors and adjusts the protein folding capacity of the endoplasmic reticulum (ER). In S. pombe , the ER membrane-resident kinase/endoribonuclease Ire1 utilizes a mechanism of selective degradation of ER-bound mRNAs (RIDD) to maintain homeostasis. We used a genetic screen to identify factors critical to the Ire1-mediated UPR and found several proteins, Dom34, Hbs1 and Ski complex subunits, previously implicated in ribosome rescue and mRNA no-go-decay (NGD). Ribosome profiling in ER-stressed cells lacking these factors revealed that Ire1-mediated cleavage of ER-associated mRNAs results in ribosome stalling and mRNA degradation. Stalled ribosomes iteratively served as a ruler to template precise, regularly spaced upstream mRNA cleavage events. This clear signature uncovered hundreds of novel target mRNAs. Our results reveal that the UPR in S. pombe executes RIDD in an intricate interplay between Ire1, translation, and the NGD pathway, and establish a critical role for NGD in maintaining ER homeostasis. |
PMC | PMC5650469 |
PMID | 28945192 |
DOI |
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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SRR5564114 | PRJNA386877 | Schizosaccharomyces pombe | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR5564115 | PRJNA386877 | Schizosaccharomyces pombe | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR5564116 | PRJNA386877 | Schizosaccharomyces pombe | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR5564117 | PRJNA386877 | Schizosaccharomyces pombe | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR5564118 | PRJNA386877 | Schizosaccharomyces pombe | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR5564119 | PRJNA386877 | Schizosaccharomyces pombe | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR5564120 | PRJNA386877 | Schizosaccharomyces pombe | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR5564121 | PRJNA386877 | Schizosaccharomyces pombe | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR5564122 | PRJNA386877 | Schizosaccharomyces pombe | 0.0 | Ribo-Seq | 0.0 | ||||||||
SRR5564123 | PRJNA386877 | Schizosaccharomyces pombe | 0.0 | Ribo-Seq | 0.0 | ||||||||
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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