Guydosh et al. 2017 (PRJNA386877)

General Details

Title Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe
Organism
Number of Samples 10
Release Date 2017/05/15 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP107240
ENA SRP107240
GEO GSE98934
BioProject PRJNA386877

Publication

Title
Authors Guydosh NR, Kimmig P, Walter P, Green R
Journal eLife
Publication Date 2017 Sep 25
Abstract The unfolded protein response (UPR) monitors and adjusts the protein folding capacity of the endoplasmic reticulum (ER). In S. pombe , the ER membrane-resident kinase/endoribonuclease Ire1 utilizes a mechanism of selective degradation of ER-bound mRNAs (RIDD) to maintain homeostasis. We used a genetic screen to identify factors critical to the Ire1-mediated UPR and found several proteins, Dom34, Hbs1 and Ski complex subunits, previously implicated in ribosome rescue and mRNA no-go-decay (NGD). Ribosome profiling in ER-stressed cells lacking these factors revealed that Ire1-mediated cleavage of ER-associated mRNAs results in ribosome stalling and mRNA degradation. Stalled ribosomes iteratively served as a ruler to template precise, regularly spaced upstream mRNA cleavage events. This clear signature uncovered hundreds of novel target mRNAs. Our results reveal that the UPR in S. pombe executes RIDD in an intricate interplay between Ire1, translation, and the NGD pathway, and establish a critical role for NGD in maintaining ER homeostasis.
PMC PMC5650469
PMID 28945192
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR5564114 PRJNA386877 Schizosaccharomyces pombe 0.0 Ribo-Seq 0.0
SRR5564115 PRJNA386877 Schizosaccharomyces pombe 0.0 Ribo-Seq 0.0
SRR5564116 PRJNA386877 Schizosaccharomyces pombe 0.0 Ribo-Seq 0.0
SRR5564117 PRJNA386877 Schizosaccharomyces pombe 0.0 Ribo-Seq 0.0
SRR5564118 PRJNA386877 Schizosaccharomyces pombe 0.0 Ribo-Seq 0.0
SRR5564119 PRJNA386877 Schizosaccharomyces pombe 0.0 Ribo-Seq 0.0
SRR5564120 PRJNA386877 Schizosaccharomyces pombe 0.0 Ribo-Seq 0.0
SRR5564121 PRJNA386877 Schizosaccharomyces pombe 0.0 Ribo-Seq 0.0
SRR5564122 PRJNA386877 Schizosaccharomyces pombe 0.0 Ribo-Seq 0.0
SRR5564123 PRJNA386877 Schizosaccharomyces pombe 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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