Song et al. 2018 (PRJNA438895)

General Details

Title Divergent rRNAs as regulators of gene expression at the ribosome level
Organism
Number of Samples 4
Release Date 2018/03/19 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP152673
ENA SRP152673
GEO GSE111991
BioProject PRJNA438895

Publication

Title
Authors Song W, Joo M, Yeom JH, Shin E, Lee M, Choi HK, Hwang J, Kim YI, Seo R, Lee JE, Moore CJ, Kim YH, Eyun SI, Hahn Y, Bae J, Lee K
Journal Nature microbiology
Publication Date 2019 Mar
Abstract It is generally assumed that each organism has evolved to possess a unique ribosomal RNA (rRNA) species optimal for its physiological needs. However, some organisms express divergent rRNAs, the functional roles of which remain unknown. Here, we show that ribosomes containing the most variable rRNAs, encoded by the rrnI operon (herein designated as I-ribosomes), direct the preferential translation of a subset of mRNAs in Vibrio vulnificus, enabling the rapid adaptation of bacteria to temperature and nutrient shifts. In addition, genetic and functional analyses of I-ribosomes and target mRNAs suggest that both I-ribosomal subunits are required for the preferential translation of specific mRNAs, the Shine-Dalgarno sequences of which do not play a critical role in I-ribosome binding. This study identifies genome-encoded divergent rRNAs as regulators of gene expression at the ribosome level, providing an additional level of regulation of gene expression in bacteria in response to environmental changes.
PMC
PMID 30718849
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR8258332 PRJNA438895 Vibrio vulnificus 0.0 Ribo-Seq 0.0
SRR8258333 PRJNA438895 Vibrio vulnificus 0.0 Ribo-Seq 0.0
SRR8258336 PRJNA438895 Vibrio vulnificus 0.0 Ribo-Seq 0.0
SRR8258337 PRJNA438895 Vibrio vulnificus 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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