Greenblatt et al. 2018 (PRJNA466150)

General Details

Title Chromatin and gene expression changes during female Drosophila germline stem cell development.
Organism
Number of Samples 11
Release Date 2023/04/18 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP144935
ENA SRP144935
GEO 0.0
BioProject PRJNA466150

Publication

Title
Authors Greenblatt EJ, Spradling AC
Journal Science (New York, N.Y.)
Publication Date 2018 Aug 17
Abstract Mutations in the fragile X mental retardation 1 gene ( FMR1 ) cause the most common inherited human autism spectrum disorder. FMR1 influences messenger RNA (mRNA) translation, but identifying functional targets has been difficult. We analyzed quiescent Drosophila oocytes, which, like neural synapses, depend heavily on translating stored mRNA. Ribosome profiling revealed that FMR1 enhances rather than represses the translation of mRNAs that overlap previously identified FMR1 targets, and acts preferentially on large proteins. Human homologs of at least 20 targets are associated with dominant intellectual disability, and 30 others with recessive neurodevelopmental dysfunction. Stored oocytes lacking FMR1 usually generate embryos with severe neural defects, unlike stored wild-type oocytes, which suggests that translation of multiple large proteins by stored mRNAs is defective in fragile X syndrome and possibly other autism spectrum disorders. Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMC PMC6905618
PMID 30115809
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR7132295 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR7132294 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR7132293 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR7132292 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR7132291 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR7132290 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR7132289 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR7132288 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR7132287 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR7132286 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
SRR7132277 PRJNA466150 Drosophila melanogaster 0.0 Ribo-Seq Cycloheximide
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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