Sharma et al. 2019 (PRJNA506724)

General Details

Title Translation complex profiling for bacteria [Ribo-seq]
Organism
Number of Samples 6
Release Date 2018/11/23 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP170541
ENA SRP170541
GEO GSE122870
BioProject PRJNA506724

Publication

Title
Authors Sharma H, Anand B
Journal Nucleic acids research
Publication Date 2019 Dec 2
Abstract In bacteria, the assembly factors tightly orchestrate the maturation of ribosomes whose competency for protein synthesis is validated by translation machinery at various stages of translation cycle. However, what transpires to the quality control measures when the ribosomes are produced with assembly defects remains enigmatic. In Escherichia coli, we show that 30S ribosomes that harbour assembly defects due to the lack of assembly factors such as RbfA and KsgA display suboptimal initiation codon recognition and bypass the critical codon-anticodon proofreading steps during translation initiation. These premature ribosomes on entering the translation cycle compromise the fidelity of decoding that gives rise to errors during initiation and elongation. We show that the assembly defects compromise the binding of initiation factor 3 (IF3), which in turn appears to license the rapid transition of 30S (pre) initiation complex to 70S initiation complex by tempering the validation of codon-anticodon interaction during translation initiation. This suggests that the premature ribosomes harbouring the assembly defects subvert the IF3 mediated proofreading of cognate initiation codon to enter the translation cycle. © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
PMC PMC6868393
PMID 31586395
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR8239838 PRJNA506724 Escherichia coli 0.0 Ribo-Seq 0.0
SRR8239839 PRJNA506724 Escherichia coli 0.0 Ribo-Seq 0.0
SRR8239840 PRJNA506724 Escherichia coli 0.0 Ribo-Seq 0.0
SRR8239841 PRJNA506724 Escherichia coli 0.0 Ribo-Seq 0.0
SRR8239842 PRJNA506724 Escherichia coli 0.0 Ribo-Seq 0.0
SRR8239843 PRJNA506724 Escherichia coli 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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