Saini et al. 2019 (PRJNA533159)
General Details
Title | Free circular introns with an unusual branchpoint in neuronal projections |
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Organism | |
Number of Samples | 3 |
Release Date | 2019/04/16 00:00 |
Sequencing Types | |
Protocol Details |
Study Links
GWIPS-viz | Trips-Viz |
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Repository Details
SRA | SRP192787 |
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ENA | SRP192787 |
GEO | |
BioProject | PRJNA533159 |
Publication
Title | |
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Authors | Saini H,Bicknell AA,Eddy SR,Moore MJ |
Journal | eLife |
Publication Date | 2019 Nov 7 |
Abstract | The polarized structure of axons and dendrites in neuronal cells depends in part on RNA localization. Previous studies have looked at which polyadenylated RNAs are enriched in neuronal projections or at synapses, but less is known about the distribution of non-adenylated RNAs. By physically dissecting projections from cell bodies of primary rat hippocampal neurons and sequencing total RNA, we found an unexpected set of free circular introns with a non-canonical branchpoint enriched in neuronal projections. These introns appear to be tailless lariats that escape debranching. They lack ribosome occupancy, sequence conservation, and known localization signals, and their function, if any, is not known. Nonetheless, their enrichment in projections has important implications for our understanding of the mechanisms by which RNAs reach distal compartments of asymmetric cells. © 2019, Saini et al. |
PMC | PMC6879206 |
PMID | 31697236 |
DOI |
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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SRR8919681 | PRJNA533159 | Rattus norvegicus | Primary hippocampal neurons | Ribo-Seq | Cycloheximide | ||||||||
SRR8919682 | PRJNA533159 | Rattus norvegicus | Primary hippocampal neurons | Ribo-Seq | Cycloheximide | ||||||||
SRR8919683 | PRJNA533159 | Rattus norvegicus | Primary hippocampal neurons | Ribo-Seq | Cycloheximide | ||||||||
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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