Lee et al. 2019 (PRJNA587444)
General Details
| Title | Recruitment of mRNAs to P granules by gelation with intrinsically-disordered proteins (RNAseq and ribosome profiling) |
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| Organism | |
| Number of Samples | 12 |
| Release Date | 2019/11/04 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP228538 |
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| ENA | SRP228538 |
| GEO | GSE139880 |
| BioProject | PRJNA587444 |
Publication
| Title | |
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| Authors | Lee CS, Putnam A, Lu T, He S, Ouyang JPT, Seydoux G |
| Journal | eLife |
| Publication Date | 2020 Jan 24 |
| Abstract | RNA granules are protein/RNA condensates. How specific mRNAs are recruited to cytoplasmic RNA granules is not known. Here, we characterize the transcriptome and assembly of P granules, RNA granules in the C. elegans germ plasm. We find that P granules recruit mRNAs by condensation with the disordered protein MEG-3. MEG-3 traps mRNAs into non-dynamic condensates in vitro and binds to ~500 mRNAs in vivo in a sequence-independent manner that favors embryonic mRNAs with low ribosome coverage. Translational stress causes additional mRNAs to localize to P granules and translational activation correlates with P granule exit for two mRNAs coding for germ cell fate regulators. Localization to P granules is not required for translational repression but is required to enrich mRNAs in the germ lineage for robust germline development. Our observations reveal similarities between P granules and stress granules and identify intrinsically-disordered proteins as drivers of RNA condensation during P granule assembly. © 2020, Lee et al. |
| PMC | PMC7007223 |
| PMID | 31975687 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR10398489 | PRJNA587444 | Caenorhabditis elegans | 0.0 | Ribo-Seq | Frozen |  |
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| SRR10398490 | PRJNA587444 | Caenorhabditis elegans | 0.0 | Ribo-Seq | Frozen | |
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| SRR10398491 | PRJNA587444 | Caenorhabditis elegans | 0.0 | Ribo-Seq | Frozen | |
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| SRR10398492 | PRJNA587444 | Caenorhabditis elegans | 0.0 | Ribo-Seq | Frozen | |
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| SRR10398493 | PRJNA587444 | Caenorhabditis elegans | 0.0 | Ribo-Seq | Frozen | |
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| SRR10398494 | PRJNA587444 | Caenorhabditis elegans | 0.0 | Ribo-Seq | Frozen | |
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| SRR10398495 | PRJNA587444 | Caenorhabditis elegans | 0.0 | RNA-Seq | Frozen | |
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| SRR10398496 | PRJNA587444 | Caenorhabditis elegans | 0.0 | RNA-Seq | Frozen | |
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| SRR10398497 | PRJNA587444 | Caenorhabditis elegans | 0.0 | RNA-Seq | Frozen | |
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| SRR10398498 | PRJNA587444 | Caenorhabditis elegans | 0.0 | RNA-Seq | Frozen | |
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| SRR10398499 | PRJNA587444 | Caenorhabditis elegans | 0.0 | RNA-Seq | Frozen | |
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| SRR10398500 | PRJNA587444 | Caenorhabditis elegans | 0.0 | RNA-Seq | Frozen | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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