Quarato et al. 2021 (PRJNA609216)

General Details

Title Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos (Ribo-Seq)
Organism
Number of Samples 7
Release Date 2020/02/27 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP251101
ENA SRP251101
GEO GSE146059
BioProject PRJNA609216

Publication

Title
Authors Quarato P, Singh M, Cornes E, Li B, Bourdon L, Mueller F, Didier C, Cecere G
Journal Nature communications
Publication Date 2021 Mar 4
Abstract Inheritance and clearance of maternal mRNAs are two of the most critical events required for animal early embryonic development. However, the mechanisms regulating this process are still largely unknown. Here, we show that together with maternal mRNAs, C. elegans embryos inherit a complementary pool of small non-coding RNAs that facilitate the cleavage and removal of hundreds of maternal mRNAs. These antisense small RNAs are loaded into the maternal catalytically-active Argonaute CSR-1 and cleave complementary mRNAs no longer engaged in translation in somatic blastomeres. Induced depletion of CSR-1 specifically during embryonic development leads to embryonic lethality in a slicer-dependent manner and impairs the degradation of CSR-1 embryonic mRNA targets. Given the conservation of Argonaute catalytic activity, we propose that a similar mechanism operates to clear maternal mRNAs during the maternal-to-zygotic transition across species.
PMC PMC7933186
PMID 33664268
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR11195102 PRJNA609216 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR11195103 PRJNA609216 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR11195104 PRJNA609216 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR11195105 PRJNA609216 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR11195106 PRJNA609216 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR11195107 PRJNA609216 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR11195108 PRJNA609216 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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