Mundodi et al. 2020 (PRJNA646470)

General Details

Title Global Translational Landscape of the Candida albicans Morphological Transition
Organism
Number of Samples 6
Release Date 2020/07/15 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP272074
ENA SRP272074
GEO GSE154488
BioProject PRJNA646470

Publication

Title
Authors Mundodi V, Choudhary S, Smith AD, Kadosh D
Journal G3 (Bethesda, Md.)
Publication Date 2021 Feb 9
Abstract Candida albicans, a major human fungal pathogen associated with high mortality and/or morbidity rates in a wide variety of immunocompromised individuals, undergoes a reversible morphological transition from yeast to filamentous cells that is required for virulence. While previous studies have identified and characterized global transcriptional mechanisms important for driving this transition, as well as other virulence properties, in C. albicans and other pathogens, considerably little is known about the role of genome-wide translational mechanisms. Using ribosome profiling, we report the first global translational profile associated with C. albicans morphogenesis. Strikingly, many genes involved in pathogenesis, filamentation, and the response to stress show reduced translational efficiency (TE). Several of these genes are known to be strongly induced at the transcriptional level, suggesting that a translational fine-tuning mechanism is in place. We also identify potential upstream open reading frames (uORFs), associated with genes involved in pathogenesis, and novel ORFs, several of which show altered TE during filamentation. Using a novel bioinformatics method for global analysis of ribosome pausing that will be applicable to a wide variety of genetic systems, we demonstrate an enrichment of ribosome pausing sites in C. albicans genes associated with protein synthesis and cell wall functions. Altogether, our results suggest that the C. albicans morphological transition, and most likely additional virulence processes in fungal pathogens, is associated with widespread global alterations in TE that do not simply reflect changes in transcript levels. These alterations affect the expression of many genes associated with processes essential for virulence and pathogenesis. © The Author(s) 2020. Published by Oxford University Press on behalf of Genetics Society of America.
PMC PMC7849906
PMID 33585865
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR12228381 PRJNA646470 Candida albicans 0.0 Ribo-Seq 0.0
SRR12228382 PRJNA646470 Candida albicans 0.0 Ribo-Seq 0.0
SRR12228383 PRJNA646470 Candida albicans 0.0 Ribo-Seq 0.0
SRR12228384 PRJNA646470 Candida albicans 0.0 Ribo-Seq 0.0
SRR12228385 PRJNA646470 Candida albicans 0.0 Ribo-Seq 0.0
SRR12228386 PRJNA646470 Candida albicans 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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