Datasets

Title	Loss of N1-Methylation of G37 in tRNA Induces Ribosome Stalling and Reprograms Gene Expression
Organism
Number of Samples	6
Release Date	2021/01/26 00:00
Sequencing Types
Protocol Details

Title
Authors	Masuda I, Hwang JY, Christian T, Maharjan S, Mohammad F, Gamper H, Buskirk AR, Hou YM
Journal	eLife
Publication Date	2021 Aug 12
Abstract	N 1 -methylation of G37 is required for a subset of tRNAs to maintain the translational reading-frame. While loss of m 1 G37 increases ribosomal +1 frameshifting, whether it incurs additional translational defects is unknown. Here, we address this question by applying ribosome profiling to gain a genome-wide view of the effects of m 1 G37 deficiency on protein synthesis. Using E coli as a model, we show that m 1 G37 deficiency induces ribosome stalling at codons that are normally translated by m 1 G37-containing tRNAs. Stalling occurs during decoding of affected codons at the ribosomal A site, indicating a distinct mechanism than that of +1 frameshifting, which occurs after the affected codons leave the A site. Enzyme- and cell-based assays show that m 1 G37 deficiency reduces tRNA aminoacylation and in some cases peptide-bond formation. We observe changes of gene expression in m 1 G37 deficiency similar to those in the stringent response that is typically induced by deficiency of amino acids. This work demonstrates a previously unrecognized function of m 1 G37 that emphasizes its role throughout the entire elongation cycle of protein synthesis, providing new insight into its essentiality for bacterial growth and survival. © 2021, Masuda et al.
PMC	PMC8384417
PMID	34382933
DOI

Masuda et al. 2021 (PRJNA695008)