Datasets

Title	Critical contribution of 3’ non-seed base pairing to the in vivo function of the evolutionarily conserved let-7a microRNA [Ribo-seq]
Organism
Number of Samples	12
Release Date	2021/04/08 00:00
Sequencing Types
Protocol Details

Title
Authors	Duan Y, Veksler-Lublinsky I, Ambros V
Journal	Cell reports
Publication Date	2022 Apr 26
Abstract	Base pairing of the seed region (g2-g8) is essential for microRNA targeting; however, the in vivo function of the 3' non-seed region (g9-g22) is less well understood. Here, we report a systematic investigation of the in vivo roles of 3' non-seed nucleotides in microRNA let-7a, whose entire g9-g22 region is conserved among bilaterians. We find that the 3' non-seed sequence functionally distinguishes let-7a from its family paralogs. The complete pairing of g11-g16 is essential for let-7a to fully repress multiple key targets, including evolutionarily conserved lin-41, daf-12, and hbl-1. Nucleotides at g17-g22 are less critical but may compensate for mismatches in the g11-g16 region. Interestingly, a certain minimal complementarity to let-7a 3' non-seed sequence can be required even for sites with perfect seed pairing. These results provide evidence that the specific configurations of both seed and 3' non-seed base pairing can critically influence microRNA-mediated gene regulation in vivo. Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
PMC	PMC9161110
PMID	35476978
DOI

Duan et al. 2021 (PRJNA720671)