Wu et al. 2022 (PRJNA763584)

General Details

Title TRL-1 undergoes phase separation to regulate the reproduction and longevity tradeoff
Organism
Number of Samples 30
Release Date 2021/09/15 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP337255
ENA SRP337255
GEO GSE184209
BioProject PRJNA763584

Publication

Title
Authors Wu D, Wang Z, Huang J, Huang L, Zhang S, Zhao R, Li W, Chen D, Ou G
Journal Proceedings of the National Academy of Sciences of the United States of America
Publication Date 2022 May 3
Abstract The antagonistic pleiotropy theory of aging proposes that genes enhancing fitness in early life limit the lifespan, but the molecular evidence remains underexplored. By profiling translatome changes in Caenorhabditis elegans during starvation recovery, we find that an open reading frame (ORF) trl-1 “hidden” within an annotated pseudogene significantly translates upon refeeding. trl-1 mutant animals increase brood sizes but shorten the lifespan and specifically impair germline deficiency–induced longevity. The loss of trl-1 abnormally up-regulates the translation of vitellogenin that produces copious yolk to provision eggs, whereas vitellogenin overexpression is known to reduce the lifespan. We show that the TRL-1 protein undergoes liquid–liquid phase separation (LLPS), through which TRL-1 granules recruit vitellogenin messenger RNA and inhibit its translation. These results indicate that trl-1 functions as an antagonistic pleiotropic gene to regulate the reproduction–longevity tradeoff by optimizing nutrient production for the next generation.
PMC PMC9170148
PMID 35482917
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR15906252 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906253 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906254 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906255 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906256 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906257 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906258 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906259 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906260 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906261 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906272 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906273 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906274 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906275 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906276 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906277 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906278 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906279 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906280 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906281 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906292 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906293 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906294 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906295 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906296 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906297 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906298 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906299 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906300 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
SRR15906301 PRJNA763584 Caenorhabditis elegans 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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