Datasets

Title	A parasitic fungus employs mutated eIF4A to survive on rocaglate-synthesizing Aglaia plants
Organism
Number of Samples	16
Release Date	2022/04/03 00:00
Sequencing Types
Protocol Details

Title
Authors	Chen M,Kumakura N,Saito H,Muller R,Nishimoto M,Mito M,Gan P,Ingolia NT,Shirasu K,Ito T,Shichino Y,Iwasaki S
Journal	eLife
Publication Date	2023 Feb 28
Abstract	Plants often generate secondary metabolites as defense mechanisms against parasites. Although some fungi may potentially overcome the barrier presented by antimicrobial compounds, only a limited number of examples and molecular mechanisms of resistance have been reported. Here, we found an Aglaia plant-parasitizing fungus that overcomes the toxicity of rocaglates, which are translation inhibitors synthesized by the plant, through an amino acid substitution in a eukaryotic translation initiation factor (eIF). De novo transcriptome assembly revealed that the fungus belongs to the Ophiocordyceps genus and that its eIF4A, a molecular target of rocaglates, harbors an amino acid substitution critical for rocaglate binding. Ribosome profiling harnessing a cucumber-infecting fungus, Colletotrichum orbiculare , demonstrated that the translational inhibitory effects of rocaglates were largely attenuated by the mutation found in the Aglaia parasite. The engineered C. orbiculare showed a survival advantage on cucumber plants with rocaglates. Our study exemplifies a plant-fungus tug-of-war centered on secondary metabolites produced by host plants. © 2023, Chen, Kumakura et al.
PMC	PMC9977294
PMID	36852480
DOI

Chen et al. 2023 (PRJNA822806)