0.0 et al. 2022 (PRJNA872137)

General Details

Title The Translation Initiation Factor Homolog, eif4e1c, Regulates Cardiomyocyte Metabolism and Proliferation During Heart Regeneration.
Organism
Number of Samples 4
Release Date 2022/08/22 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP394145
ENA SRP394145
GEO GSE211793
BioProject PRJNA872137

Publication

Title
Authors Rao A, Lyu B, Jahan I, Lubertozzi A, Zhou G, Tedeschi F, Jankowsky E, Kang J, Carstens B, Poss KD, Baskin K, Goldman JA
Journal Development (Cambridge, England)
Publication Date 2023 Oct 15
Abstract The eIF4E family of translation initiation factors bind 5' methylated caps and act as the limiting step for mRNA translation. The canonical eIF4E1A is required for cell viability, yet other related eIF4E families exist and are utilized in specific contexts or tissues. Here, we describe a family called Eif4e1c, for which we find roles during heart development and regeneration in zebrafish. The Eif4e1c family is present in all aquatic vertebrates but is lost in all terrestrial species. A core group of amino acids shared over 500 million years of evolution forms an interface along the protein surface, suggesting that Eif4e1c functions in a novel pathway. Deletion of eif4e1c in zebrafish caused growth deficits and impaired survival in juveniles. Mutants surviving to adulthood had fewer cardiomyocytes and reduced proliferative responses to cardiac injury. Ribosome profiling of mutant hearts demonstrated changes in translation efficiency of mRNA for genes known to regulate cardiomyocyte proliferation. Although eif4e1c is broadly expressed, its disruption had most notable impact on the heart and at juvenile stages. Our findings reveal context-dependent requirements for translation initiation regulators during heart regeneration. © 2023. Published by The Company of Biologists Ltd.
PMC PMC10281269
PMID 37306388
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR21218378 PRJNA872137 Danio rerio WtNA Ribo-Seq 0.0
SRR21218377 PRJNA872137 Danio rerio WtNA Ribo-Seq 0.0
SRR21218374 PRJNA872137 Danio rerio MutantNA Ribo-Seq 0.0
SRR21218373 PRJNA872137 Danio rerio MutantNA Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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