FastQCFastQC Report
Thu 22 Jun 2023
DRR244662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR244662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50599092
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG17523983.463299301892611TruSeq Adapter, Index 9 (100% over 49bp)
AGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGC17421243.442994589705286No Hit
AAAAGGTCGACGCGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC11356802.2444671536793583Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCA7076661.3985745040642232No Hit
GACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGCACA6627951.3098950471285138No Hit
ACGATGCCGACCAGGGATCAGCGGATGTAGATCGGAAGAGCACACGTCTG6564931.2974402781773238Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGGTCGACGCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5449091.076914581787357Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCAGATCGGAAGAGCACAC4850770.9586674005928802No Hit
TGCCGGCCGGGGGACGGACTGGGAAGATCGGAAGAGCACACGTCTGAACT4420790.8736895911096587Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAG4222580.8345169514109068No Hit
GACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCACAC3713090.7338254212150684No Hit
ACGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAGCACACGTCTGA3359870.6640178444308842Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCCGGCCGGGGGACGGACTGGGAAAGATCGGAAGAGCACACGTCTGAAC3246570.641626138271414Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCCGGCCGGGGGACGGACTGGGAACAGATCGGAAGAGCACACGTCTGAA3174070.6272978179134123Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACCAGGGATCAGCGGATGTAGATCGGAAGAGCACACGTCT3168750.6262464156471425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCCGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGT2946400.5823029393491883No Hit
CGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAGCACACGTCTGAA2853140.5638717785686748Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCAACCATAAACGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGA2759220.545310180664902No Hit
CTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAA2577870.509469616569404No Hit
GGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGCAC2470540.48825777347941346No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCAGATCGGAAGAGCACACG2469340.48802061507348793No Hit
GTCTAGTGGTAGAATAGTACCCTAGATCGGAAGAGCACACGTCTGAACTC2467370.4876312800237601Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAG2401000.4745144438560281No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCAGATCGGAAG2344750.4633976435782681No Hit
GTGCCGGCCGGGGGACGGACTGGGAAAGATCGGAAGAGCACACGTCTGAA2339300.46232054915135634Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAAGATCGGA2177560.43035554867269166No Hit
CGATGCCGACCAGGGATCAGCGGATGTAGATCGGAAGAGCACACGTCTGA2134920.42192851998213726Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGCA2086190.4122979123815107No Hit
CGCCGCGTGCCGGCCGGGGGACGGACTGGGAAGATCGGAAGAGCACACGT2079120.41090065410659943No Hit
TGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAG1911840.3778407723205784No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCAGATCGGAAGAGCACACGT1831640.36199068552455443No Hit
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCAGATCGGAAGAGCACA1808340.35738585980950016No Hit
TGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAA1788430.3534510065911855No Hit
ATGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAA1722150.34035195730389783No Hit
CTCAACCATAAACGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAG1693440.33467794244212923No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTAGATCGGAAGAGCACA1688920.33378464577980965No Hit
AAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGC1648800.32585564974169895No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAAGATCGGAA1643870.3248813239573548No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACG1578290.3119206170735238No Hit
TTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCAGATCGGAA1527490.3018809112226757No Hit
GGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCACA1527040.3017919768204536No Hit
CGCCGCGTGCCGGCCGGGGGACGGACTGGGAAAGATCGGAAGAGCACACG1521360.300669427032406No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGCACAC1478850.2922680905024936No Hit
GGGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCAC1285830.25412116090936965No Hit
GTGCCGGCCGGGGGACGGACTGGGAACAGATCGGAAGAGCACACGTCTGA1129480.22322139693732052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTAGATCGGAA1086180.21466393112350712No Hit
ATGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGA1081510.21374098966044688No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCACACG1074060.2122686312236591No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCAGATCGGAAGAGCAC1005080.19863597552303905No Hit
ACGATGCCGACCAGGGATCAGCGGATGTTAGATCGGAAGAGCACACGTCT978800.1934422064332696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTAGATCGGAAGAGCACACG934710.1847286113355552No Hit
AACGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAGCACACGTCTG932000.18419302860217332Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGCACACGTCTGAACTC877760.1734734686543387Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCAGATCGGAAGAGCACAC825340.16311359895549116No Hit
CCGCGTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCAGATCGGAAGAG797250.15756211593678401No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACAAAAGATCGGAAGAGCACAC775240.15321223550809965No Hit
GTGCCGGCCGGGGGACGGACTGGGAAGATCGGAAGAGCACACGTCTGAAC774330.1530323903836061Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGTCGGCTTGTCCCTTCGGTCGGCAGATCGGAAGAGCACACGTCTGAAC761170.15043155319862261Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCAGCGGAAGCGTGGTGGGCAGATCGGAAGAGCACACGTCTGAACTCCA747720.14777340273220713Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCAGATCGGAAGAGCACACG716960.14169424226031566No Hit
AACGATGCCGACCAGGGATCAGCGGATGTTAGATCGGAAGAGCACACGTC694670.13728902487024866No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGAGATCGGAAGAGCACACGT692850.13692933462126158No Hit
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTAGATCGGAAGAGCAC686620.13569808723049814No Hit
CGTGCCGGCCGGGGGACGGACTGGGAAAGATCGGAAGAGCACACGTCTGA679130.13421782351351286Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGTGCCGGCCGGGGGACGGACTGGGAAAGATCGGAAGAGCACACGTC656880.12982051140364337No Hit
GCCGCGTGCCGGCCGGGGGACGGACTGGGAAGATCGGAAGAGCACACGTC656560.1297572691620632No Hit
TTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTAGATCGGA644950.12746276158473357No Hit
GAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGA643440.12716433725727727No Hit
GCGCAGCGGAAGCGTGGTGGGCAGATCGGAAGAGCACACGTCTGAACTCC607060.11997448491763449Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAAAGGTCGACGCGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT603380.11924719913946281Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCACACGTCTGAACTCC576160.1138676559650517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTCC565080.11167789335033917Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTAGATCGGAAGA562330.11113440533675979No Hit
CCCTTCACGGCCGGCTTCTTAGAAGATCGGAAGAGCACACGTCTGAACTC557120.11010474259103306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTCAGATCGGAAGAGCAC551780.10904938768466438No Hit
TGAACGGGGCTAAGCGATCTGCCGAAGCTGTGGGATGTCAGATCGGAAGA551600.10901381392377554No Hit
CCGCGTGCCGGCCGGGGGACGGACTGGGAAGATCGGAAGAGCACACGTCT544030.10751773964639524Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAG512820.1013496447722817No Hit
CGCGTGGTGTCCGGTGCGCCCCCGGCGGCCAGATCGGAAGAGCACACGTC506190.10003934457954305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)