FastQCFastQC Report
Fri 23 Jun 2023
DRR244663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR244663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50768935
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGC20650634.0675720300219025No Hit
AGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCA7213461.420841307780043No Hit
ACGATGCCGACCAGGGATCAGCGGATGTAGATCGGAAGAGCACACGTCTG6945451.368051151752543Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCAGATCGGAAGAGCACAC5841671.150638673038936No Hit
AAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAG5675111.1178312091833322No Hit
GACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGCACA4780550.9416289705505936No Hit
AAAAGGTCGACGCGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC4136620.8147935346683951Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAGCACACGTCTGA3160840.6225933240474711Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3160320.6224908992083447TruSeq Adapter, Index 10 (100% over 49bp)
AACGATGCCGACCAGGGATCAGCGGATGTAGATCGGAAGAGCACACGTCT3124630.6154610097690645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCCGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGT3107260.6120396262005496No Hit
ATGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAA2989340.5888128242201653No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCAGATCGGAAG2893190.5698740775239819No Hit
TGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAA2857660.5628757034198176No Hit
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCAGATCGGAAGAGCACA2822230.5558970264001007No Hit
TGCCGGCCGGGGGACGGACTGGGAACAGATCGGAAGAGCACACGTCTGAA2821030.5556606613867319Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCCGGCCGGGGGACGGACTGGGAAAGATCGGAAGAGCACACGTCTGAA2756700.5429895269617139Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCAGATCGGAAGAGCACACG2711620.5341100812928221No Hit
TCTCAACCATAAACGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGA2601080.5123369241446566No Hit
TTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCAGATCGGAA2522590.49687668256188555No Hit
TGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAG2461200.4847846424196214No Hit
GTCTAGTGGTAGAATAGTACCCTAGATCGGAAGAGCACACGTCTGAACTC2455290.4836205447287795Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGA2424010.4774592967136301No Hit
TGCCGGCCGGGGGACGGACTGGGAAGATCGGAAGAGCACACGTCTGAACT2382950.46937167383952416Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCCGGCCGGGGGACGGACTGGGAAAGATCGGAAGAGCACACGTCTGAAC2284920.4500626219557294Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCACAC2227340.4387210407309115No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTAGATCGGAAGAGCACA2165070.42645566624551023No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCAGATCGGAAGAGCACACGT2151960.42387337847445483No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAG1888140.37190853028530146No Hit
CTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAA1885420.37137276958833193No Hit
AAAAGGTCGACGCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1883030.37090200927003886Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGC1838700.3621702917345026No Hit
CGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAGCACACGTCTGAA1800160.35457903538847135Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAAGATCGGA1750970.344890039548791No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTAGATCGGAA1727310.3402297093685341No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACG1725700.33991258630893084No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGCACAC1704480.33573286498919075No Hit
TACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAAGATCGGAA1571440.3095278638403583No Hit
GGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGCAC1540730.3034788892065591No Hit
CGATGCCGACCAGGGATCAGCGGATGTAGATCGGAAGAGCACACGTCTGA1515410.29849158742447524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCCGGCCGGGGGACGGACTGGGAACAGATCGGAAGAGCACACGTCTGA1488160.2931241319125564Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGCA1364080.2686839895302118No Hit
TTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTAGATCGGA1357440.2673761031229038No Hit
CGCCGCGTGCCGGCCGGGGGACGGACTGGGAAGATCGGAAGAGCACACGT1250980.24640658702019258No Hit
ACGATGCCGACCAGGGATCAGCGGATGTTAGATCGGAAGAGCACACGTCT1185020.2334143901186818Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTAGATCGGAAGAGCAC1133960.223357058799835No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTAGATCGGAAGAGCACACG1123970.22138932006353887No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCAGATCGGAAGAGCAC1118550.2203217380864893No Hit
CTCAACCATAAACGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAG1112210.2190729429325236No Hit
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCAGATCGGAAGAGCACACG1108380.21831854459818784No Hit
ACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCACACG1037080.2042745233871855No Hit
TTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCAGATCGGAAGAGCACAC986440.19429991982301775No Hit
CGCCGCGTGCCGGCCGGGGGACGGACTGGGAAAGATCGGAAGAGCACACG951680.1874532132690985No Hit
AACGATGCCGACCAGGGATCAGCGGATGAGATCGGAAGAGCACACGTCTG863340.17005280886825774Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCACA822580.16202427724749396No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGAGATCGGAAGAGCACACGT805780.15871516706032932No Hit
AACGATGCCGACCAGGGATCAGCGGATGTTAGATCGGAAGAGCACACGTC784550.15453347603214446No Hit
GTGCCGGCCGGGGGACGGACTGGGAAGATCGGAAGAGCACACGTCTGAAC773660.15238846353582167Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGCCGAGGGCACGTCTGCCTGGGTGTCACAAAAGATCGGAAGAGCACAC760910.14987708526877705No Hit
TGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTCAGATCGGAAGAGCAC734190.14461402430442946No Hit
GGGACGCATTTATTAGATAAAAGGTCGACGCGGGCAGATCGGAAGAGCAC718650.14155309738130217No Hit
TAGATAAAAGGTCGACGCGGGCTAGATCGGAAGAGCACACGTCTGAACTC701780.13823019923502433Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTAGATCGGAAGA655260.12906711555008984No Hit
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTCAGATCGGAAGAGCA648610.12775725943433716No Hit
TTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTAGATCGGAAG629250.12394390388531884No Hit
GAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTAGATCGGAAGA623330.12277783648603226No Hit
CGTGCCGGCCGGGGGACGGACTGGGAAAGATCGGAAGAGCACACGTCTGA623180.12274829085936113Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCAGATCGGAAGAG615810.12129661573558712No Hit
TTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAGATCGGAAGAG612320.12060918748837257No Hit
ATTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCAGATCGGA587780.11577552296497848No Hit
TTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCAGATCGGAAGAGCA568020.11188337907817053No Hit
TGAACGGGGCTAAGCGATCTGCCGAAGCTGTGGGATGTCAGATCGGAAGA551600.10864911781190605No Hit
CCGCGTGCCGGCCGGGGGACGGACTGGGAAAGATCGGAAGAGCACACGTC539310.10622834613331952No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCAGATCGGAAGAGCACACGTCTGAAC530190.10443197203171585Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCTAGATCGGAAGAGCACAC516990.10183195688465792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)