FastQCFastQC Report
Thu 22 Jun 2023
DRR277023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51445285
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length10-89
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCT27944065.431801962026258No Hit
ACTGTGGTAATTCTAGAGCT22110874.297939062831511No Hit
TTGGTGACTCTAGATAACCTC21882364.253520998085635No Hit
AACTGTGGTAATTCTAGAGCT19780053.8448713035606668No Hit
TTGGTGACTCTAGATAACCTCG13847102.691616928548457No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT10629322.0661407551731905No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT7529551.4636035158518415No Hit
CTGTGGTAATTCTAGAGCT7230651.4055029532832795No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG6976981.3561942557029278No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA6211611.2074206606105884No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA5933771.1534137676562586No Hit
TAACTGTGGTAATTCTAGAGCT5318101.0337390491665077No Hit
TTTGGTGACTCTAGATAACCT4779130.9289733743335273No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT4541800.8828408667577602No Hit
TTTGGTGACTCTAGATAACCTC4401210.8555128035543005No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA4087910.794613150651221No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3942180.7662859677033571No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3890310.7562034110608971No Hit
GAGGGCGCACCACCGGCCCGTCTCG3310160.6434331153962896No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT3261950.6340619942138527No Hit
TTTGGTGACTCTAGATAACCTCG3180820.6182918415166715No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2833870.5508512587693897No Hit
ACTGTGGTAATTCTAGAGC2597500.5049053572159237No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA2276150.44244093506333965No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2132120.41444420027996737No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2066550.40169862019425107No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT1887630.36691992278787067No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC1821870.3541374102602406No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC1804610.3507823894842841No Hit
TTGGTGACTCTAGATAACC1793580.348638364040553No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC1720920.33451462072763327No Hit
TTGGTGACTCTAGATAACCTCGG1713010.3329770648563809No Hit
TGGTGACTCTAGATAACCT1678790.3263253376864372No Hit
TGGTGACTCTAGATAACCTC1553970.30206266716182056No Hit
CAGTGGCTGGTTGAGAT1547420.30078946982216154No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT1494620.2905261385955973No Hit
AACTGTGGTAATTCTAGAGC1476410.28698645561007197No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG1262660.24543745845707726No Hit
CACTCGCCGAATCCCGGGGCCGAGGG1192520.2318035559526981No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC1178440.22906666762561428No Hit
AACTGTGGTAATTCTAGAGCTA1106740.2151295303349957No Hit
AGGAATAATGGAAT1101380.21408764671048086No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG935420.18182813060516625No Hit
TAGGAATAATGGAAT914460.1777538991182574No Hit
TGGTGACTCTAGATAACCTCG908720.17663815060991497No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT895400.17404899205048627No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC877490.17056762344693008No Hit
GGTTGGCCTCGGATAGCCGGTC847640.1647653424410031No Hit
TTTCTAGAGCT840620.16340078590292578No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA807220.15690845137703097No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC805210.15651774501783788No Hit
AAACTGTGGTAATTCTAGAGCT783420.1522821770741478No Hit
CGAGGGCGCACCACCGGCCCGTCTCG773600.15037335297102544No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCA768660.14941310948126732No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGC765200.1487405502758902No Hit
TTTTGGTGACTCTAGATAACCTCG725560.14103527660503776No Hit
AGCTAGGAATAATGGAAT720970.1401430665609103No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA713970.1387823976482976No Hit
CGCTTCTGGCGCCAAGCGCCCGG712770.13854914012042113No Hit
AAGGAATAATGGAAT705770.13718847120780844No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA684400.13303454339887513No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG663650.12900113197934465No Hit
TTTTGGTGACTCTAGATAACCTC660760.12843937009970885No Hit
AGGGAGTCGGGTTCAGATCCCCGAATCCGGAG611640.11889136195863237No Hit
TTTGGTGACTCTAGATAACCTCGG598370.11631192246286516No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA593420.11534973516037475No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCT583130.11334955185883411No Hit
CCTGCGGAAGGATCATTA566720.11015975516512348No Hit
TAACTGTGGTAATTCTAGAGCTA565120.10984874512795487No Hit
TGTGGTAATTCTAGAGCT554440.10777275312985436No Hit
CAACTGTGGTAATTCTAGAGCT551940.10728679994677841No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG544250.10579200795563676No Hit
TTGGTGACTCTAGATAACCTCGGGCCG517290.10055148882934559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)