FastQCFastQC Report
Thu 22 Jun 2023
DRR277024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52552922
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length10-89
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCG23269934.427904123009563No Hit
TTGGTGACTCTAGATAACCT21584524.1071969318851576No Hit
AACTGTGGTAATTCTAGAGCT21078874.010979636869668No Hit
TTGGTGACTCTAGATAACCTC20991423.994339268138126No Hit
ACTGTGGTAATTCTAGAGCT20882723.97365535640435No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT9483641.8045885250681208No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG8677431.6511793578290472No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA7270551.3834720741122635No Hit
TAACTGTGGTAATTCTAGAGCT6069931.1550128459079783No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC5981261.1381403302370132No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA5952791.132722934035904No Hit
CTGTGGTAATTCTAGAGCT5859831.1150340983894291No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT5384411.0245691000778223No Hit
TTTGGTGACTCTAGATAACCTCG5323391.012957947419175No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA4879610.9285135467824226No Hit
GAGGGCGCACCACCGGCCCGTCTCG4707220.8957104231045421No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4513590.8588656592681945No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4202110.7995958816524037No Hit
TTTGGTGACTCTAGATAACCTC3939370.7496005645509112No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT3873930.7371483549477991No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT3671280.6985872260347389No Hit
TTTGGTGACTCTAGATAACCT3359300.6392223062306602No Hit
GAGGGCGCACCACCGGCCCGTCTCGC3289970.6260298904026688No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC3091720.5883060127465415No Hit
TTGGTGACTCTAGATAACCTCGG1982480.3772349708737413No Hit
ACTGTGGTAATTCTAGAGC1949530.37096510066557287No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1728220.3288532652856106No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC1714420.3262273408888663No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC1659000.31568178073904246No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC1519890.2892113211136005No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG1463930.2785630074004258No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT1430180.2721409096909968No Hit
AACTGTGGTAATTCTAGAGC1360330.258849545987186No Hit
TGGTGACTCTAGATAACCTCG1299100.24719843360945754No Hit
TGGTGACTCTAGATAACCTC1261980.24013507755096852No Hit
TTTTGGTGACTCTAGATAACCTCG1256350.2390637765108475No Hit
TTGGTGACTCTAGATAACC1227140.23350556987107207No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG1155170.2198108032889208No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC1146710.21820099746309063No Hit
AACTGTGGTAATTCTAGAGCTA1113110.211807442410148No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG1109020.21102917930995352No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC1085760.2066031647108033No Hit
TGGTGACTCTAGATAACCT1015090.19315576781820049No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC1008860.19197029615213404No Hit
CACTCGCCGAATCCCGGGGCCGAGGG990560.1884880920607992No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT917060.1745021903824872No Hit
TTTCTAGAGCT890150.16938163780883583No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGC872510.16602502140604095No Hit
CGAGGGCGCACCACCGGCCCGTCTCG824020.15679813198588652No Hit
AAACTGTGGTAATTCTAGAGCT803320.1528592453907701No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT783470.14908210051574297No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA766120.14578066658215502No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG765830.14572548411294808No Hit
CCTGCGGAAGGATCATTA762180.14503094613844686No Hit
GGTTGGCCTCGGATAGCCGGTC757750.14418798635021665No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA736340.1401139978477315No Hit
TTTGGTGACTCTAGATAACCTCGG718960.1368068553828463No Hit
AGGAATAATGGAAT698600.13293266547576554No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCA673650.12818507028020248No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC660190.12562384257149392No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG659940.12557627147735No Hit
CAGTGGCTGGTTGAGAT650120.1237076788993769No Hit
TTGGTGACTCTAGATAACCTCGGGCCG636010.12102276634589414No Hit
TTTTGGTGACTCTAGATAACCTC632300.1203168113087984No Hit
AGGGAGTCGGGTTCAGATCCCCGAATCCGGAG628530.11959943920910811No Hit
CAACTGTGGTAATTCTAGAGCT623590.11865943438882427No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG612130.11647877543326705No Hit
TAGGAATAATGGAAT589760.11222211392926924No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATA570980.10864857333717809No Hit
TAACTGTGGTAATTCTAGAGCTA561000.10674953525895287No Hit
CGCTTCTGGCGCCAAGCGCCCGG553980.10541373893539165No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGC543230.10336818188720315No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG539530.10266412969387316No Hit
CTAAACCATTCGTAGACGACCTGCTT538300.10243007991068509No Hit
TAGATAACCTCG529160.10069088070878342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)