FastQCFastQC Report
Thu 22 Jun 2023
DRR277025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27733936
Total Bases676.1 Mbp
Sequences flagged as poor quality0
Sequence length10-88
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATT12389304.467198597415094No Hit
AACTGTGGTAATTCTAGAGCT8779643.16566678454872No Hit
TTGGTGACTCTAGATAACCTCG8032832.896390184213305No Hit
TTGGTGACTCTAGATAACCTC8021522.8923121478321723No Hit
TTGGTGACTCTAGATAACCT7315972.6379126280525056No Hit
ACTGTGGTAATTCTAGAGCT7015462.5295580115278264No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT5949452.145187758419865No Hit
CAGTGGCTGGTTGAGAT3959351.4276192171208588No Hit
TAACTGTGGTAATTCTAGAGCT3476961.253684294937437No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG3076641.109341277776079No Hit
TTTGGTGACTCTAGATAACCTCG2765790.9972583768852716No Hit
TTTGGTGACTCTAGATAACCTC2751830.9922248324219108No Hit
GAGGGCGCACCACCGGCCCGTCTCG2523210.9097915276071886No Hit
TTTGGTGACTCTAGATAACCT2186870.7885177206726084No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1930180.6959632415680198No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT1587610.5724430892174843No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1580270.569796512114256No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1576380.5683938983633624No Hit
CTGTGGTAATTCTAGAGCT1562940.5635478498255712No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1461260.5268851849950184No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1438330.5186173358155871No Hit
AGCAGTGGCTGGTTGAGATTT1311750.4729765007029655No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1283580.4628192695043358No Hit
ATTGATCATCGACACTTCGAACGCA1231930.44419587612807643No Hit
ATTGATCATCGACACTTCGAACG1214330.4378498601857306No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1197150.4316552832601907No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1165120.4201062553832965No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1134200.40895745919367527No Hit
TTGGTGACTCTAGATAACCTCGG1130800.407731524295722No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1086740.39184485029459937No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG899220.3242309349816052No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG878430.3167347036497091No Hit
AGAGCAGTGGCTGGTTGAGATTTAA876320.3159739028748029No Hit
CGCTTCTGGCGCCAAGCGCCCGG837090.30182877756694904No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT826370.2979634769475202No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT820460.29583251363960744No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC819910.2956342006414091No Hit
AACGATGCCGACCGGCGATGCGGCGGCG766810.27648798208808156No Hit
AACTGTGGTAATTCTAGAGCTA757130.2729976733197913No Hit
ACTGTGGTAATTCTAGAGC748340.2698282710394947No Hit
CAGCTAGGAATAATGGAAT703070.25350530844233576No Hit
AGCTAGGAATAATGGAAT685520.24717732095437156No Hit
AACTGTGGTAATTCTAGAGC671500.24212214234575288No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA648260.23374251674915525No Hit
AGGAATAATGGAAT647050.23330622815311897No Hit
CGAGGGCGCACCACCGGCCCGTCTCG636030.22933275680740015No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG633230.22832316336202693No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATT630770.22743616340644904No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT579740.2090363228645224No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGG575690.20757601805960757No Hit
AAACTGTGGTAATTCTAGAGCT568750.20507366859143253No Hit
CACTCGCCGAATCCCGGGGCCGAGGG561210.20235497767067753No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG554680.20000046152843218No Hit
TGGTGACTCTAGATAACCTCG532810.19211481558189217No Hit
ATTGATCATCGACACTTCGAACGC532650.19205712452787085No Hit
GTTGAACCCCATTCGTGATGGGGATCGGG532050.19184078307529084No Hit
TTGGTGACTCTAGATAACC526140.18970981976737814No Hit
TGGTGACTCTAGATAACCTC501970.18099486491928157No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC484820.17481110506637065No Hit
TAACTGTGGTAATTCTAGAGCTA457560.16498199173748726No Hit
TTTGGTGACTCTAGATAACCTCGG450000.1622560894349796No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC444100.16012873181794318No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC427650.1541973703263756No Hit
TTTTGGTGACTCTAGATAACCTCG417110.15039697214272074No Hit
TTTTGGTGACTCTAGATAACCTC409950.1478152974752664No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG409530.14766385845846042No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT406270.1464884032327759No Hit
CAACTGTGGTAATTCTAGAGCT385690.13906789140928283No Hit
TTGATCATCGACACTTCGAACGCA378720.13655472486847883No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA377190.1360030541643999No Hit
TTGATCATCGACACTTCGAACG369770.13332763153416088No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG363690.1311353714813505No Hit
GTTGAACCCCATTCGTGATGGGGATCGG363190.13095508693753385No Hit
TGGTGACTCTAGATAACCT358630.12931089189792605No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA352720.12717992859001334No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC351100.1265958066680474No Hit
TCGCTTCTGGCGCCAAGCGCCCGG345860.12470642464884897No Hit
TAGGAATAATGGAAT341590.12316679464465484No Hit
GGTTGGCCTCGGATAGCCGGTC335260.12088439231993611No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC333390.12021012812606187No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCG328790.11855151032294875No Hit
ACGATGCCGACCGGCGATGCGGCGGCG326240.11763205914948385No Hit
TCAGTGGCTGGTTGAGAT323850.11677029903004031No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC317030.11431121785238128No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG316860.11424992110748361No Hit
AACGATGCCGACCGGCGATGCGGCGGC313910.11318624229896543No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG312660.11273553093942382No Hit
TTGGTGACTCTAGATAACCTCGGGCCG311760.11241101876055386No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC308460.11122114077136401No Hit
TTAACTGTGGTAATTCTAGAGCT308160.11111297004507402No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC307300.1108028806297094No Hit
CAGTGGCTGGTTGAGA303330.10937142135180523No Hit
CAGTGGCTGGTTGAGATTT303130.10929930753427858No Hit
CTAAACCATTCGTAGACGACCTGCTT302750.10916229128097793No Hit
TTTGATCATCGACACTTCGAACGCA299100.10784621411111643No Hit
AGGGCGCACCACCGGCCCGTCTCG296300.10683662066574323No Hit
AGGGCGCACCACCGGCCCGTCTCGC294100.10604336867294999No Hit
CCGGGGTCCGGTGCGGAGTGCCCT290570.10477055979360447No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG285370.1028956005379114No Hit
AGTGGCTGGTTGAGAT284580.1026107509586811No Hit
TCGGTTGGCCTCGGATAGCCGGTC283730.10230426723419281No Hit
ATTGATCATCGACACTTCGAAC282650.10191485261954886No Hit
TTTCTAGAGCT278890.10055911285004769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)