FastQCFastQC Report
Thu 22 Jun 2023
DRR277026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26330536
Total Bases634.9 Mbp
Sequences flagged as poor quality0
Sequence length10-88
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCG11884024.513398435945247No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT11002084.178448930929473No Hit
AACTGTGGTAATTCTAGAGCT9523743.616994352108897No Hit
TTGGTGACTCTAGATAACCTC8151173.0957098632553475No Hit
TTGGTGACTCTAGATAACCT7947673.018423172243816No Hit
ACTGTGGTAATTCTAGAGCT7634672.899549785086031No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT5509182.0923159331051977No Hit
TTTGGTGACTCTAGATAACCTCG3964731.5057536238533085No Hit
TAACTGTGGTAATTCTAGAGCT3839791.4583030136568431No Hit
GAGGGCGCACCACCGGCCCGTCTCG3436151.3050057165566247No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2756791.0469934983473181No Hit
TTTGGTGACTCTAGATAACCTC2564400.9739262428991192No Hit
TTTGGTGACTCTAGATAACCT2118740.8046702885197629No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2074150.7877355781895211No Hit
CTGTGGTAATTCTAGAGCT2005590.7616973691686337No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT1459950.5544702925910814No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1285910.48837213188519973No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1258660.4780229312460635No Hit
TTGGTGACTCTAGATAACCTCGG1204860.45759038099338356No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1189800.4518707860713508No Hit
ATTGATCATCGACACTTCGAACG1131490.4297253956394963No Hit
CAGCTAGGAATAATGGAAT1129310.4288974595883654No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1114430.4232462263586279No Hit
GGTTGGCCTCGGATAGCCGGTC1058090.40184901667022654No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1044110.39653959190196514No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC933960.3547060340890896No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC921690.3500460453976326No Hit
ACTGTGGTAATTCTAGAGC918640.3488876945004082No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC866800.32919952711938716No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGG865380.3286602293246138No Hit
ATTGATCATCGACACTTCGAACGCA863820.328067761324722No Hit
GTTGAACCCCATTCGTGATGGGGATCGGG840370.3191617519673735No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT808880.30720225368750564No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG795070.3019573927397452No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT793070.30119781838090953No Hit
AACTGTGGTAATTCTAGAGCTA781660.29686444666375195No Hit
AACGATGCCGACCGGCGATGCGGCGGCG765780.29083342625459657No Hit
TGGTGACTCTAGATAACCTCG759800.28856229892167784No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG748190.2841529697686367No Hit
AACTGTGGTAATTCTAGAGC723600.2748140030267519No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC717840.2726264288733051No Hit
CACTCGCCGAATCCCGGGGCCGAGGG661540.2512444106720805No Hit
AAACTGTGGTAATTCTAGAGCT611080.23208034959865612No Hit
GTTGAACCCCATTCGTGATGGGGATCGG600560.2280849884711804No Hit
AGCTAGGAATAATGGAAT589810.22400227629243855No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATT581200.22073230867765092No Hit
AGGGCGCACCACCGGCCCGTCTCG574720.21827128775502327No Hit
TTTTGGTGACTCTAGATAACCTCG569770.21639134121690498No Hit
TGGTGACTCTAGATAACCTC569280.21620524549899023No Hit
TTGGTGACTCTAGATAACC542770.20613708737262318No Hit
AGGAATAATGGAAT527750.20043268393776717No Hit
CGAGGGCGCACCACCGGCCCGTCTCG526290.1998781946558171No Hit
TGGTGACTCTAGATAACCT524820.1993199075020729No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT516360.19610690796419794No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA501770.19056581301649156No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG492500.18704518586328817No Hit
TTTGGTGACTCTAGATAACCTCGG469220.17820374032644076No Hit
CAGTGGCTGGTTGAGAT464100.1762592299678214No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCC459280.17442865576302738No Hit
TAACTGTGGTAATTCTAGAGCTA452800.17196763484039976No Hit
ATTGATCATCGACACTTCGAACGC445470.16918379481526696No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG442550.16807481625136683No Hit
CAACTGTGGTAATTCTAGAGCT440840.16742538017456232No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCG425680.16166780653458784No Hit
CGCTTCTGGCGCCAAGCGCCCGG422310.1603879237399497No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC402220.1527579993054452No Hit
TTGAACCCCATTCGTGATGGGGATCGGGG394580.1498564252546929No Hit
CCGGGGTCCGGTGCGGAGTGCCCT391430.14866009563952667No Hit
ACGATGCCGACCGGCGATGCGGCGGCG388080.1473878085884769No Hit
TTGATCATCGACACTTCGAACG385710.1464877129732566No Hit
TTGAACCCCATTCGTGATGGGGATCGGG370360.1406579797691927No Hit
CGGTTGGCCTCGGATAGCCGGTC370140.14057442658972077No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT356630.1354435017957857No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA353010.1340686722062931No Hit
TTTTGGTGACTCTAGATAACCTC337120.12803385392534355No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG337050.12800726882278432No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA334530.12705020513065135No Hit
GTTGGCCTCGGATAGCCGGTC332260.12618808823337282No Hit
TTAACTGTGGTAATTCTAGAGCT331040.12572474787448307No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC328470.12474869482337922No Hit
CCTGCGGAAGGATCATTA328200.1246461522849364No Hit
TTGGTGACTCTAGATAACCTCGGGCCG318870.1211027379009679No Hit
GTTGAACCCCATTCGTG317650.12063939754207813No Hit
AACGATGCCGACCGGCGATGCGGCGGC312800.11879742972190159No Hit
AGGGCGCACCACCGGCCCGTCTCGC309100.11739221715805558No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC299840.11387538787664633No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC299110.1135981432356713No Hit
TTTCTAGAGCT297440.11296389864604352No Hit
CAGCTAGGAATAATGGAA288370.10951922892872368No Hit
TTGATCATCGACACTTCGAACGCA285650.10848620780070713No Hit
CTAGGAATAATGGAAT283450.10765067600598788No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC280860.10666702721129566No Hit
GTTGAACCCCATTCG278830.10589605923707744No Hit
AGATAACCTCG270330.10266786821202577No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC264620.10049928341754988No Hit
GTTGAACCCCATTCGTGATGGGGATCG264580.10048409193037315No Hit
CTAAACCATTCGTAGACGACCTGCTT264070.10029040046887006No Hit
TTGAACCCCATTCGTGATGGGGATCGG263640.10012709198172039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)