FastQCFastQC Report
Thu 22 Jun 2023
DRR277027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55523279
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC30104155.421897002876937No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC7353251.3243544207826774No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA6685731.204130973604783No Hit
TCGGTTGGCCTCGGATAGCCGGTC4462580.8037313502323953No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT4071240.733249201654679No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3774730.6798463757877125No Hit
TTCGGTTGGCCTCGGATAGCCGGTC3699880.6663655437208599No Hit
CGGTTGGCCTCGGATAGCCGGTC3580070.6447872071820542No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT3398810.6121414407099407No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT3191650.5748309641438863No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2937540.5290645748785838No Hit
CCGGGGTCCGGTGCGGAGTGCCCT2470380.4449268927362881No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTT2332750.4201390915691417No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2209140.3978763574103756No Hit
TCCGGGGTCCGGTGCGGAGTGCCCT1857360.3345191482657211No Hit
CAGTGGCTGGTTGAGAT1814260.32675663841827496No Hit
TTTTGGTGACTCTAGATAACCTC1757760.3165807264372841No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1753380.3157918681279613No Hit
TTAGATAACCTCGGGCCGATCGCAC1721620.3100717448621866No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1700750.306312961091509No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1612490.29041692584474343No Hit
GGTTGGCCTCGGATAGCCGGTC1596240.2874902254962283No Hit
ATAACCTCGGGCCGATCGCAC1371000.24692345709625688No Hit
AGCTAGGAATAATGGAAT1341580.24162477868066834No Hit
TAAGGCTGCGTTCAGGTCGCAGTC1327360.2390636907449216No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1273630.2293866686079545No Hit
TTTGGTGACTCTAGATAACCTCG1262610.22740191551006922No Hit
TCAGTGGCTGGTTGAGAT1261830.22726143389334047No Hit
AGATAACCTCGGGCCGATCGCAC1236330.22266876565413218No Hit
TCGGGCCGATCGCAC1233870.22222570824752624No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1187010.2137860049655929No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1183260.21311061257747402No Hit
ACCTCGGGCCGATCGCAC1157510.20847291817905783No Hit
GTTGGCCTCGGATAGCCGGTCCCC1119850.20169017755597612No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1100830.19826458736343722No Hit
TTCGGGCCGATCGCAC1096200.19743070289490647No Hit
TTTGGTGACTCTAGATAACCTC1070380.19278040117191206No Hit
TAGGAATAATGGAAT1056890.19035078962105245No Hit
TAGCAGTGGCTGGTTGAGATTT1039420.18720436161560272No Hit
TCTAGATAACCTCGGGCCGATCGCAC995020.17920771574027536No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG991250.17852872125941985No Hit
TCGATGCCGACCGGCGATGCGGCGGCG956840.17233132070604115No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG928410.167210945880916No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT911570.16417798379667023No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA877620.1580634313762341No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT873420.15730699190154096No Hit
TTAACCTCGGGCCGATCGCAC872550.1571503008674974No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC840380.1513563347006217No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTT833360.1500920001500632No Hit
TTAGGAATAATGGAAT805980.14516073519361133No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC789240.14214578357304872No Hit
CGGTTGGCCTCGGATAGCCGGTCCCC787800.14188643289601105No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC776830.13991068502996734No Hit
TAACCTCGGGCCGATCGCAC773900.13938297844405045No Hit
GTTGGCCTCGGATAGCCGGTC740090.13329364067277077No Hit
CTAGATAACCTCGGGCCGATCGCAC738510.13300907534657672No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCT733040.13202390298310732No Hit
GGTTGGCCTCGGATAGCCGGTCCCC701380.12632179018101578No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC699800.1260372248548217No Hit
TAACTGTGGTAATTCTAGAGCT694870.12514930899524143No Hit
TTTTGGTGACTCTAGATAACC682150.12285837801474225No Hit
ATGCGTGCATTTATCAGA661750.11918424342337562No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC649930.11705540661602497No Hit
CTGCGTTCAGGTCGCAGTC615340.11082558722801657No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA613620.11051580725266605No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC610660.10998269752764422No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT599380.10795111722418267No Hit
CTCGGTTGGCCTCGGATAGCCGGTC590530.10635719118822215No Hit
TTGGTGACTCTAGATAACCTCG590390.10633197653906572No Hit
TCGGGGTCCGGTGCGGAGTGCCCTT590280.10631216502901422No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG561200.10107472218994847No Hit
AAGGAATAATGGAAT555520.10005172785274444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)