FastQCFastQC Report
Thu 22 Jun 2023
DRR277028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51151886
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC23113844.518668187522938No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC21484194.200077784033223No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC10944292.139567248800953No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA5422451.0600684400962264No Hit
TCGGTTGGCCTCGGATAGCCGGTC5371861.0501782866813554No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA4923440.9625138748549761No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT4041790.7901546386774477No Hit
TTGGTGACTCTAGATAACCTCG3804490.7437633873362949No Hit
AACTGTGGTAATTCTAGAGCT3458570.6761373373407972No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3084680.6030432582681311No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA2758530.5392821684033312No Hit
TTGGTGACTCTAGATAACCTC2532350.4950648349505627No Hit
CGGTTGGCCTCGGATAGCCGGTC2466860.48226178796222685No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2326290.45478088530303656No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2283740.4464625214405584No Hit
CAGTGGCTGGTTGAGAT2261090.44203453221646605No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1957070.38259977354500674No Hit
TCGGGCCGATCGCAC1937420.37875827295986697No Hit
TAACTGTGGTAATTCTAGAGCT1886230.3687508218172053No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1817620.3553378266443587No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT1671990.3268677131474683No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1656930.3239235401799261No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1629500.31856107905776926No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1581410.3091596661753586No Hit
CCGGGGTCCGGTGCGGAGTGCCCT1579740.308833187499675No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1459930.28541078622203686No Hit
ATAACCTCGGGCCGATCGCAC1388490.2714445367664449No Hit
ACTGTGGTAATTCTAGAGCT1368300.2674974682262938No Hit
AGATAACCTCGGGCCGATCGCAC1355410.2649775220409273No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT1349860.26389251805886493No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1322460.2585359218230976No Hit
TTTGGTGACTCTAGATAACCTC1305550.2552300808615346No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1262580.24682960858960312No Hit
ACCTCGGGCCGATCGCAC1252220.24480426782308673No Hit
TTTGGTGACTCTAGATAACCTCG1119190.21879740660979735No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC1118250.21861364016959217No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1094190.2139100012851921No Hit
AACCTCGGGCCGATCGCAC1072340.20963840903148712No Hit
AGCTAGGAATAATGGAAT1049810.2052338793529529No Hit
TTTTGGTGACTCTAGATAACCTC980360.19165666736119955No Hit
ATTGATCATCGACACTTCGAACGCA968170.18927356852492205No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT919390.17973726325555228No Hit
TTAGATAACCTCGGGCCGATCGCAC914640.1788086562438773No Hit
AGGAATAATGGAAT896410.17524476028117517No Hit
CTAGATAACCTCGGGCCGATCGCAC881740.1723768308366968No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA880870.17220674913140055No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG839930.16420313417182703No Hit
TAGATAACCTCGGGCCGATCGCAC825110.16130588029540102No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC753280.14726338731674526No Hit
AGCAGTGGCTGGTTGAGATTT744020.14545309238451148No Hit
TTGGTGACTCTAGATAACCT741090.14488028848046774No Hit
GAGGGCGCACCACCGGCCCGTCTCGC739890.14464569302488672No Hit
TAGGAATAATGGAAT704270.13768211791838916No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC667470.13048785728057025No Hit
CCTGCGGAAGGATCATTA640400.12519577479508773No Hit
TCGCTTCTGGCGCCAAGCGCCCGG607580.11877958908494597No Hit
GGTTGGCCTCGGATAGCCGGTC596950.11670146434092381No Hit
TTGGTGACTCTAGATAACCTCGG585410.11444543804308603No Hit
TAACCTCGGGCCGATCGCAC549810.10748577286084818No Hit
TTCGGTTGGCCTCGGATAGCCGGTC547860.10710455524552898No Hit
AGAGCAGTGGCTGGTTGAGATTTAA540930.10574976648954841No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC528120.10324546000122067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)