FastQCFastQC Report
Thu 22 Jun 2023
DRR277031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20236423
Total Bases531.3 Mbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC8051243.978588508453297No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2366801.1695742869181969No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1785420.882280430686787No Hit
TCGGTTGGCCTCGGATAGCCGGTC1579310.780429426682769No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT1410980.6972477299965513No Hit
TTCGGTTGGCCTCGGATAGCCGGTC1410480.6970006507573003No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1255500.6204159697590824No Hit
CAGTGGCTGGTTGAGAT1111850.5494301043222906No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT1062410.5249989091451587No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1019220.5036562044586634No Hit
CGGTTGGCCTCGGATAGCCGGTC948510.4687142584437971No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT899370.44443131081021586No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTT772100.38153976125128436No Hit
AACGATGCCGACCGGCGATGCGGCGGCG741910.3666211167853133No Hit
ACGATGCCGACCGGCGATGCGGCGGCG731860.3616548240763696No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC631080.3118535326129524No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC616090.3044460970202095No Hit
CCGGGGTCCGGTGCGGAGTGCCCT614240.3035319038349811No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT594620.29383651448677467No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA590710.29190435483583244No Hit
TCGATGCCGACCGGCGATGCGGCGGCG571140.28223367341155103No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT567440.2804052870410942No Hit
TTTGGTGACTCTAGATAACCTCG563010.27821616498133095No Hit
TCAGTGGCTGGTTGAGAT561370.2774057450765879No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA519930.2569278177274709No Hit
ATGCGTGCATTTATCAGA512820.2534143509453227No Hit
TAGCAGTGGCTGGTTGAGATTT485490.23990899972786695No Hit
TCCGGGGTCCGGTGCGGAGTGCCCT466780.23066329459509716No Hit
TTAGATAACCTCGGGCCGATCGCAC435670.21529002432890434No Hit
GGTTGGCCTCGGATAGCCGGTC422910.20898456214322067No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTT414990.20507082699348597No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC397760.19655647640889895No Hit
AGCTAGGAATAATGGAAT394920.19515306632995366No Hit
GTTGGCCTCGGATAGCCGGTCCCC393340.19437229593392075No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC392240.1938287216075687No Hit
AGATAACCTCGGGCCGATCGCAC359730.17776362947147328No Hit
ATAACCTCGGGCCGATCGCAC358350.17708169077114072No Hit
TAAGGCTGCGTTCAGGTCGCAGTC357430.176627064970919No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT356090.17596489260972653No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT343750.1698669769850136No Hit
AAGCAGTGGTATCAACGCAGAGTGGGGG328390.16227670275522507Clontech Universal Primer Mix Long (100% over 23bp)
TCGGGCCGATCGCAC320960.15860510525995627No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA305530.15098023993667262No Hit
CGGTTGGCCTCGGATAGCCGGTCCCC301440.14895913175960002No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC298140.14732840878054387No Hit
AGAGCAGTGGCTGGTTGAGATTTAA296410.14647351461273564No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCT285130.1408994069752347No Hit
TAGGAATAATGGAAT279330.13803328779992394No Hit
GGTTGGCCTCGGATAGCCGGTCCCC279030.13788504025637338No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA278970.13785539074766326No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT278510.1376280778475524No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA277730.13724263423432095No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCC271750.13428756653287985No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG269640.13324489214324092No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG268200.13253330393419827No Hit
TTGGTGACTCTAGATAACCTCG266150.13152027905326943No Hit
ACCTCGGGCCGATCGCAC265060.13098164631170242No Hit
TTTTGGTGACTCTAGATAACCTC265000.1309519968029923No Hit
CTCGGTTGGCCTCGGATAGCCGGTC263800.13035900662879008No Hit
TTCGGGCCGATCGCAC262360.12964741841974742No Hit
AGCAGTGGCTGGTTGAGATTT247110.12211150162259407No Hit
TCTAGATAACCTCGGGCCGATCGCAC244110.12062902618708851No Hit
TTAACCTCGGGCCGATCGCAC241390.11928491512556345No Hit
GTTGGCCTCGGATAGCCGGTC235520.11638420485675753No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC234740.11599876124352608No Hit
TAGAGCAGTGGCTGGTTGAGATTTAA231960.11462500067329093No Hit
TTAGGAATAATGGAAT228660.1129942776942348No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT219110.10827506422454207No Hit
TCAGCTAGGAATAATGGAAT219110.10827506422454207No Hit
TCGGGGTCCGGTGCGGAGTGCCCTT217800.10762771661770461No Hit
AGTGGCTGGTTGAGAT217650.10755359284592934No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC203410.10051677611206289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)