FastQCFastQC Report
Thu 22 Jun 2023
DRR277032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59673248
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC30481125.108004176343812No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC27064174.535394151831655No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC13780412.309311200891897No Hit
TCGGTTGGCCTCGGATAGCCGGTC5841640.9789378315723655No Hit
AACGATGCCGACCGGCGATGCGGCGGCG5475200.917530079810638No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT4436070.7433934214541162No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA4401030.737521443444808No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT4136960.6932687826880146No Hit
ACGATGCCGACCGGCGATGCGGCGGCG3824410.640891878384096No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3704530.6208024741673187No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3431740.5750885220794417No Hit
TTGGTGACTCTAGATAACCTCG2768370.4639214543843835No Hit
ATGCGTGCATTTATCAGA2715950.45513694846977326No Hit
TCGGGCCGATCGCAC2690200.4508217819817684No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA2336320.3915188259905008No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA2212220.37072223720753394No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA2059910.34519823690508683No Hit
AGATAACCTCGGGCCGATCGCAC1966140.32948432771750585No Hit
ATAACCTCGGGCCGATCGCAC1865310.31258730880544666No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1856010.31102882149133226No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT1817660.3046021560616241No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1790300.3000171869310684No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT1730950.29007135659852135No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT1548790.25954511475561043No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT1533170.25692752638502264No Hit
ACCTCGGGCCGATCGCAC1523070.25523497564603825No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG1440190.24134600482950083No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC1416680.23740621593113218No Hit
AACTGTGGTAATTCTAGAGCT1404020.23528466223256359No Hit
AACCTCGGGCCGATCGCAC1388360.2326603706907323No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1358570.22766818390713373No Hit
CGGTTGGCCTCGGATAGCCGGTC1346200.22559522820008054No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1318310.22092144205054834No Hit
CCGGGGTCCGGTGCGGAGTGCCCT1318270.22091473887930485No Hit
CAGTGGCTGGTTGAGAT1256210.2105147686950105No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC1121290.1879049720906762No Hit
CCTGCGGAAGGATCATTA1110800.18614706543206766No Hit
TAGATAACCTCGGGCCGATCGCAC1106140.18536614598219958No Hit
TTAGATAACCTCGGGCCGATCGCAC1103800.1849740104644547No Hit
TTGGTGACTCTAGATAACCTC1091090.18284407780183173No Hit
TCGATGCCGACCGGCGATGCGGCGGCG1050250.17600013996221556No Hit
CTAGATAACCTCGGGCCGATCGCAC1040370.17434445666507042No Hit
ATTGATCATCGACACTTCGAACGCA1033660.17321999968897286No Hit
TTTGGTGACTCTAGATAACCTC1024280.1716481060323715No Hit
TAACTGTGGTAATTCTAGAGCT984540.16498850540195162No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA960340.16093308679963256No Hit
TTTGGTGACTCTAGATAACCTCG941500.15777589314394283No Hit
TTTTGGTGACTCTAGATAACCTC922780.1546388090019836No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT885250.14834955858276727No Hit
CGATGCCGACCGGCGATGCGGCGGCG881900.14778816799112393No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC861800.14441982444126386No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC823830.138056839138369No Hit
AGCTAGGAATAATGGAAT822280.13779709125268327No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG775470.12995270510497434No Hit
TAACCTCGGGCCGATCGCAC697650.11691168545073999No Hit
GCGGTCGGCGTC697050.11681113788208747No Hit
ACGATGCCGACCGGCGATGCGGCGGCGT675880.1132634845014637No Hit
AGGAATAATGGAAT672670.11272555500917263No Hit
CTCGGTTGGCCTCGGATAGCCGGTC658060.1102772217124833No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC638380.10697926146068001No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG635850.10655528587952846No Hit
ATCCCACTCCTGACACCA633740.1062016935964337No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT630710.10569392837473837No Hit
ATCCCACTTCTGACACCA606680.10166699825020417No Hit
GCAGCTAGGAATAATGGAAT600160.10057438133751326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)