FastQCFastQC Report
Thu 22 Jun 2023
DRR277035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28658398
Total Bases731.7 Mbp
Sequences flagged as poor quality0
Sequence length10-39
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC11033203.8499011703305954No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2917981.0181936896821657No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2506760.8747034638851761No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT1720630.6003929459001861No Hit
TCGGTTGGCCTCGGATAGCCGGTC1651040.576110360390696No Hit
TTCGGTTGGCCTCGGATAGCCGGTC1523390.5315684428696956No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1516100.5290246858878853No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1501380.5238883206242024No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT1410750.49226408259107857No Hit
TAGGAATAATGGAAT1347960.4703542745131811No Hit
AGATAACCTCGGGCCGATCGCAC1224610.4273127897798055No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1188520.41471962249948513No Hit
CGGTTGGCCTCGGATAGCCGGTC1166460.4070220533611125No Hit
AGCTAGGAATAATGGAAT1015700.35441618195127306No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTT1014310.35393115832922695No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC912410.31837439064109585No Hit
TAACCTCGGGCCGATCGCAC877060.30603943737538997No Hit
CCGGGGTCCGGTGCGGAGTGCCCT863900.301447415169543No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT834710.2912619191065739No Hit
TTAGATAACCTCGGGCCGATCGCAC747110.2606949627819392No Hit
ATAACCTCGGGCCGATCGCAC710050.24776332577975924No Hit
TTTTGGTGACTCTAGATAACCTC694760.24242806593725164No Hit
ATGCGTGCATTTATCAGA672960.23482122064185165No Hit
GGTTGGCCTCGGATAGCCGGTC664650.2319215470453024No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA664420.23184129133805736No Hit
TCGGGCCGATCGCAC642900.22433214864278178No Hit
TCCGGGGTCCGGTGCGGAGTGCCCT629080.2195098274509273No Hit
ACCTCGGGCCGATCGCAC609640.21272647549943302No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC604840.21105157378301467No Hit
AGGAATAATGGAAT591660.20645257282001597No Hit
TTTGGTGACTCTAGATAACCTCG589680.20576167586199343No Hit
TAAGGCTGCGTTCAGGTCGCAGTC585910.20444618013888985No Hit
GTTGGCCTCGGATAGCCGGTCCCC565060.19717082580819764No Hit
TTAGGAATAATGGAAT563060.19647295009302337No Hit
AACGATGCCGACCGGCGATGCGGCGGCG512590.17886205642059966No Hit
ACGATGCCGACCGGCGATGCGGCGGCG493190.17209266198340883No Hit
TTCGGGCCGATCGCAC489110.17066899552445325No Hit
AGGAATAATGGAATA488680.17051895224569077No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT487170.16999205608073417No Hit
TTAACCTCGGGCCGATCGCAC481880.16814617481409813No Hit
TCGATGCCGACCGGCGATGCGGCGGCG477020.16645033682622454No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTT459920.16048349946148419No Hit
GTTGGCCTCGGATAGCCGGTC452110.15775829479372852No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG447410.1561182868630689No Hit
TCTAGATAACCTCGGGCCGATCGCAC414090.1444916774482649No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC404440.14112442712254886No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC399940.13955420676340666No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT398330.13899241681269134No Hit
TCGGGGTCCGGTGCGGAGTGCCCTT387410.1351820154078396No Hit
CAGTGGCTGGTTGAGAT382550.13348617741996605No Hit
TTTGGTGACTCTAGATAACCTC380010.13259987526169467No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT369130.12880343137114644No Hit
GGTTGGCCTCGGATAGCCGGTCCCC359430.12541873415255103No Hit
AACCTCGGGCCGATCGCAC349590.12198518563389343No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA348700.12167463094064086No Hit
TAGATAACCTCGGGCCGATCGCAC347170.12114075601853251No Hit
CGGTTGGCCTCGGATAGCCGGTCCCC346920.1210535215541357No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC346490.12090347827537323No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA337460.11775256942136124No Hit
CTGCGTTCAGGTCGCAGTC326610.1139665936665406No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCT318290.11106343069141547No Hit
CGGGGTCCGGTGCGGAGTGCCCTT318250.11104947317711199No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA299590.10453829275453567No Hit
TCTAGGAATAATGGAAT298540.10417190800406917No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC296680.10352288358895707No Hit
CTAGATAACCTCGGGCCGATCGCAC296600.10349496856035009No Hit
TAGGAATAATGGAATA295210.10300994493830395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)