FastQCFastQC Report
Thu 22 Jun 2023
DRR277036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43479066
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC18076654.157552510442612No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC16170133.7190610304278384No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC7901141.8172285485617377No Hit
TCGGTTGGCCTCGGATAGCCGGTC5556951.2780748326102498No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3413150.7850099631855018No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT3351680.7708721249899895No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3008030.691834088616347No Hit
TTGGTGACTCTAGATAACCTCG2060110.473816525865574No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2055750.47281374443508056No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT1973670.4539356940188181No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1963180.4515230386963694No Hit
ATGCGTGCATTTATCAGA1883150.4331164795490317No Hit
CGGTTGGCCTCGGATAGCCGGTC1844480.4242225442469256No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT1733120.3986102185359731No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1697160.39033957169181144No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1610530.37041504065427716No Hit
CCGGGGTCCGGTGCGGAGTGCCCT1573760.3619580972599549No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1522570.35018461528129424No Hit
AACTGTGGTAATTCTAGAGCT1518830.34932443121018286No Hit
TCGGGCCGATCGCAC1414760.3253887744506747No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1396720.3212396512841375No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1355840.3118374253945565No Hit
CAGTGGCTGGTTGAGAT1320140.3036265774430389No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1316610.3028146924775247No Hit
AGATAACCTCGGGCCGATCGCAC1312300.3018234108340782No Hit
ATAACCTCGGGCCGATCGCAC1282580.294987937413375No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1246040.2865838930394687No Hit
ACCTCGGGCCGATCGCAC1098980.25276071937699857No Hit
ATTGATCATCGACACTTCGAACGCA1066190.24521915903161304No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1040660.2393473677654437No Hit
TTGGTGACTCTAGATAACCTC1037870.23870567964822428No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1033120.23761319987876464No Hit
AACCTCGGGCCGATCGCAC1010880.2324980946002842No Hit
TAACTGTGGTAATTCTAGAGCT950750.21866845069763No Hit
TTTGGTGACTCTAGATAACCTC863880.1986887206822704No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC835230.19209934270437182No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC808490.18594925659166645No Hit
AGCTAGGAATAATGGAAT796050.1830881095743869No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT776040.17848589479820012No Hit
TTTTGGTGACTCTAGATAACCTC747560.17193561609626112No Hit
TTAGATAACCTCGGGCCGATCGCAC722920.16626852103952738No Hit
TAGATAACCTCGGGCCGATCGCAC718030.16514384186633632No Hit
GCGGTCGGCGTC711370.16361207023168345No Hit
GGTTGGCCTCGGATAGCCGGTC709120.16309457981457098No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG698080.16055542683460589No Hit
TTTGGTGACTCTAGATAACCTCG693070.15940314817250215No Hit
GTTGGCCTCGGATAGCCGGTC689200.15851306465506873No Hit
CTAGATAACCTCGGGCCGATCGCAC663050.15249867602951728No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA653660.15033901602210129No Hit
GTTGGCCTCGGATAGCCGGTCCCC614320.14129098357356618No Hit
AGGAATAATGGAAT588850.1354329920518532No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA588310.1353087943517462No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT580310.13346882842423524No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC577490.13282024043478763No Hit
TAACCTCGGGCCGATCGCAC558720.12850322037736506No Hit
TTCGGTTGGCCTCGGATAGCCGGTC558700.1284986204625463No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA529570.12179884452899702No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC527790.12138945211012582No Hit
GTCAGGATGGCCGAGCGGT517950.11912629401928734No Hit
ACTGTGGTAATTCTAGAGCT514440.11831900896859193No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG506480.11648824287071852No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC500920.1152094665510984No Hit
CCTGCGGAAGGATCATTA499720.11493347166197176No Hit
GTCCGGTGCGGAGTGCCCTTC488190.11228162076894661No Hit
TAGGAATAATGGAAT476570.10960907025923694No Hit
GTCCGGTGCGGAGTGCCCTT468080.10765640641866593No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC467550.10753450867596834No Hit
CTCGGTTGGCCTCGGATAGCCGGTC459840.10576124151332965No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT448950.10325658789450537No Hit
TGCGTGCATTTATCAGA445190.10239180390857522No Hit
CGGGGTCCGGTGCGGAGTGCCCTT444870.10231820527147478No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC442850.10185361387477826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)