FastQCFastQC Report
Thu 22 Jun 2023
DRR277039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35608687
Total Bases894 Mbp
Sequences flagged as poor quality0
Sequence length10-36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC13174923.699917382519608No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3874251.0880069798698278No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3694841.0376232069438562No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1957200.5496411591924184No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1908720.5360265038696878No Hit
TAGGAATAATGGAAT1654260.4645664132462958No Hit
AGCTAGGAATAATGGAAT1559300.43789876329896693No Hit
TCGGTTGGCCTCGGATAGCCGGTC1557770.4374690928648956No Hit
CGGTTGGCCTCGGATAGCCGGTC1540500.43261915273652185No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT1528800.42933343765244697No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1459460.4098606612481948No Hit
TTTTGGTGACTCTAGATAACCTC1310440.3680113226303458No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT1309860.3678484410278874No Hit
AGATAACCTCGGGCCGATCGCAC1305190.3665369632977481No Hit
CAGTGGCTGGTTGAGAT1303500.36606236000782616No Hit
TTTGGTGACTCTAGATAACCTCG1277480.35875515432512295No Hit
CCGGGGTCCGGTGCGGAGTGCCCT1212780.34058543074053815No Hit
TTCGGTTGGCCTCGGATAGCCGGTC1172170.32918091026495866No Hit
GGTTGGCCTCGGATAGCCGGTC1116280.3134853020556473No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1107880.311126327123491No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1093790.30716942750514786No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1017750.285815087762152No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTT937190.26319139484137677No Hit
TAACCTCGGGCCGATCGCAC905880.2543985966121132No Hit
TCCGGGGTCCGGTGCGGAGTGCCCT898350.2522839440836445No Hit
TAAGGCTGCGTTCAGGTCGCAGTC895730.251548168569091No Hit
TTTGGTGACTCTAGATAACCTC878490.24670665335118927No Hit
GTTGGCCTCGGATAGCCGGTC876060.24602423560295836No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC855630.24028687157153536No Hit
ATAACCTCGGGCCGATCGCAC817850.22967710098381328No Hit
TCGGGCCGATCGCAC813780.22853412146311378No Hit
TTAGATAACCTCGGGCCGATCGCAC806630.22652618446729025No Hit
ACCTCGGGCCGATCGCAC765500.21497563221019636No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG735050.20642434808112975No Hit
TCAGTGGCTGGTTGAGAT708310.19891494454709885No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT707880.1987941874970004No Hit
TAACTGTGGTAATTCTAGAGCT704700.1979011469869698No Hit
TTAGGAATAATGGAAT663610.18636182794383854No Hit
TAGCAGTGGCTGGTTGAGATTT625400.175631300306018No Hit
AGGAATAATGGAAT620730.17431982257587875No Hit
ACGATGCCGACCGGCGATGCGGCGGCG611820.17181762416569865No Hit
TTGGTGACTCTAGATAACCTCG595900.16734680500856433No Hit
TTCGGGCCGATCGCAC595080.16711652412233002No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT587710.16504680444971195No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA579040.16261200532330777No Hit
AACGATGCCGACCGGCGATGCGGCGGCG543850.15272958533966724No Hit
TTAACCTCGGGCCGATCGCAC541680.15212018348219353No Hit
GTTGGCCTCGGATAGCCGGTCCCC539740.15157537260500506No Hit
GTCCGGTGCGGAGTGCCCTT527680.1481885585952664No Hit
AGGAATAATGGAATA518010.14547292911979598No Hit
TCTAGATAACCTCGGGCCGATCGCAC508090.142687092057059No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG461290.1295442317207596No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC460340.1292774428891467No Hit
TTTTGGTGACTCTAGATAACC445780.12518855300674242No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA427690.12010833199213439No Hit
TAGCTAGGAATAATGGAAT414130.11630027245879636No Hit
TCAGCTAGGAATAATGGAAT411170.11546901462556032No Hit
TTGGTGACTCTAGATAACCTC404010.11345826932624614No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT402850.11313250612132932No Hit
ATCGGGCCGATCGCAC401110.11264386131395408No Hit
CTGCGTTCAGGTCGCAGTC398900.11202322624251773No Hit
TCGATGCCGACCGGCGATGCGGCGGCG389500.10938342096129522No Hit
AACCTCGGGCCGATCGCAC383730.10776302984718308No Hit
TTAACTGTGGTAATTCTAGAGCT373390.10485924403783829No Hit
AGAGCAGTGGCTGGTTGAGATTTAA369400.10373873094506406No Hit
TAGATAACCTCGGGCCGATCGCAC368130.10338207640174994No Hit
TCTAGGAATAATGGAAT362190.10171394412829655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)