FastQCFastQC Report
Thu 22 Jun 2023
DRR277040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49228232
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC16658303.3838915848125524No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC16590223.3700621220766163No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC7961811.6173260091891986No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3875660.7872840121497762No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3793130.7705192418854286No Hit
TTGGTGACTCTAGATAACCTCG3710790.7537930673602091No Hit
AACTGTGGTAATTCTAGAGCT3692900.7501589738181131No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT3525210.7160951870057003No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2729330.5544237298629778No Hit
GCGGTCGGCGTC2578040.5236913647437105No Hit
TCGGTTGGCCTCGGATAGCCGGTC2535220.5149931039570952No Hit
TTGGTGACTCTAGATAACCTC2466670.5010681675506852No Hit
CCGGGGTCCGGTGCGGAGTGCCCT2359190.4792351673324364No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2200240.44694678451990716No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT2137080.4341167482919151No Hit
CAGTGGCTGGTTGAGAT2063520.41917410318534293No Hit
TAACTGTGGTAATTCTAGAGCT2011950.40869840704415306No Hit
CGGTTGGCCTCGGATAGCCGGTC2000680.406409070307461No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1915560.3891181791781594No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1862630.37836621879900134No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1697430.34480823930463317No Hit
TCGGGCCGATCGCAC1668290.338888871735227No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT1664690.33815758404648777No Hit
TTTGGTGACTCTAGATAACCTC1310340.2661765305729444No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1284440.2609153219234036No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1194020.24254781280790258No Hit
TCGCTTCTGGCGCCAAGCGCCCGG1177990.23929155123832194No Hit
TTTGGTGACTCTAGATAACCTCG1130110.2295654249780898No Hit
ACTGTGGTAATTCTAGAGCT1101460.2237455937885399No Hit
AGATAACCTCGGGCCGATCGCAC1096070.2226506936101219No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1088050.22102154714798614No Hit
ATAACCTCGGGCCGATCGCAC1079990.2193842752670866No Hit
TTTTGGTGACTCTAGATAACCTC1005400.20423240062734735No Hit
GGTTGGCCTCGGATAGCCGGTC1001050.2033487613367874No Hit
GTCCGGTGCGGAGTGCCCTT989990.2011020830486051No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT982920.19966591528210886No Hit
AGCTAGGAATAATGGAAT956640.19432751515431226No Hit
ATGCGTGCATTTATCAGA952360.19345809534658892No Hit
GCGGTCGGCGT949750.19292791177225296No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG917920.18646210979098335No Hit
GTTGGCCTCGGATAGCCGGTC911310.18511938434027042No Hit
CTAGATAACCTCGGGCCGATCGCAC893560.18151372976384772No Hit
TTAGATAACCTCGGGCCGATCGCAC887280.1802380390179359No Hit
ACCTCGGGCCGATCGCAC877040.1781579318144109No Hit
AACGATGCCGACCGGCGATGCGGCGGCG818790.16632529073967148No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC815490.1656549436916605No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT807900.16411314548123523No Hit
AGCAGTGGCTGGTTGAGATTT806410.16381047363228482No Hit
TAGATAACCTCGGGCCGATCGCAC799820.16247181089095378No Hit
ACGATGCCGACCGGCGATGCGGCGGCG790170.1605115536141944No Hit
ATTGATCATCGACACTTCGAACGCA746820.15170563102895915No Hit
TTGGTGACTCTAGATAACCT744770.15128920331731596No Hit
TCGCTTCTGGCGCCAAGCGC735450.14939598074535768No Hit
GTCCGGTGCGGAGTGCCCTTC727200.14772011312533018No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA717410.14573141688289762No Hit
CTGCGTTCAGGTCGCAGTC662500.13457724827493298No Hit
TACCCTACGCCGGCAGGCGCGGGT646780.13138395870077155No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC644810.1309837818266559No Hit
AACCTCGGGCCGATCGCAC631890.12835927156595833No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT631080.12819473183599198No Hit
GTTGGCCTCGGATAGCCGGTCCCC631010.1281805123531554No Hit
AGGAATAATGGAAT630280.1280322234607166No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC617650.12546662248605636No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC605500.12299852653656139No Hit
CGCTTCTGGCGCCAAGCGCCCGG595820.12103217519572915No Hit
AGAGCAGTGGCTGGTTGAGATTTAA595420.12095092100809146No Hit
TGCGTGCATTTATCAGA568060.11539313457367309No Hit
CAGCTAGGAATAATGGAAT559620.11367867121451772No Hit
TAGGAATAATGGAAT559580.11367054579575395No Hit
CCTGCGGAAGGATCATTA555310.11280315734272155No Hit
CGGGCCGATCGCAC518550.10533589749881735No Hit
TTGGTGACTCTAGATAACCTCGG510110.10362143413966196No Hit
CGGGGTCCGGTGCGGAGTGCCCTT501180.10180743440065042No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC494470.10044439540302808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)