FastQCFastQC Report
Thu 22 Jun 2023
DRR277043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62063402
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC28850314.64852216770199No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC6922111.1153288052111612No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA6759961.0892022967094197No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT3575780.5761495317320826No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3554260.5726821098205348No Hit
TCGGTTGGCCTCGGATAGCCGGTC3228550.5202019057866019No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3063080.49354046044720523No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT2905320.46812129312537526No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT2875580.46332941916397047No Hit
CGGTTGGCCTCGGATAGCCGGTC2743730.4420850149335997No Hit
TTCGGTTGGCCTCGGATAGCCGGTC2602130.41926963655650074No Hit
CCGGGGTCCGGTGCGGAGTGCCCT2193850.3534852955691988No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTT2098390.3381042502310782No Hit
TCGGGCCGATCGCAC1936570.3120309131619952No Hit
TTTGGTGACTCTAGATAACCTCG1898850.3059532572835759No Hit
TAGGAATAATGGAAT1880250.30295632198827904No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1824820.29402513255718726No Hit
GGTTGGCCTCGGATAGCCGGTC1762340.28395800797384585No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1750710.2820841177865177No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1702670.2743436461958692No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1673180.269592053622842No Hit
TCCGGGGTCCGGTGCGGAGTGCCCT1629380.26253475437907836No Hit
TTAGATAACCTCGGGCCGATCGCAC1599680.25774932543981394No Hit
TAAGGCTGCGTTCAGGTCGCAGTC1578230.25429318231701187No Hit
AGCTAGGAATAATGGAAT1523650.24549894960640412No Hit
ATAACCTCGGGCCGATCGCAC1388560.22373249858265906No Hit
TTAGGAATAATGGAAT1386480.22339735743135705No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1378320.2220825729147107No Hit
CAGTGGCTGGTTGAGAT1363420.21968180216740293No Hit
TTTTGGTGACTCTAGATAACCTC1327340.213868392196741No Hit
TTCGGGCCGATCGCAC1296010.20882032860525435No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1253740.20200955145836189No Hit
AGATAACCTCGGGCCGATCGCAC1246430.20083172366219948No Hit
ACCTCGGGCCGATCGCAC1177580.18973822930299566No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1136050.18304668506570104No Hit
GTTGGCCTCGGATAGCCGGTCCCC1046380.1685985566824068No Hit
TAACTGTGGTAATTCTAGAGCT1041300.16778003887057302No Hit
GTTGGCCTCGGATAGCCGGTC1034080.1666167123742266No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1025990.16531320664632596No Hit
TTTGGTGACTCTAGATAACCTC1022750.16479115985295167No Hit
TCTAGATAACCTCGGGCCGATCGCAC971680.15656247783516605No Hit
TTAACCTCGGGCCGATCGCAC951240.15326907152140967No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG926100.14921837510615354No Hit
TTGGTGACTCTAGATAACCTCG886300.14280557807643224No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA866660.13964107220548433No Hit
ATGCGTGCATTTATCAGA864260.13925437087705891No Hit
TCGATGCCGACCGGCGATGCGGCGGCG861730.13884672322667713No Hit
AACGATGCCGACCGGCGATGCGGCGGCG860050.13857603229677937No Hit
TAGCAGTGGCTGGTTGAGATTT831740.13401456787689467No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC816420.1315461243971125No Hit
TCAGTGGCTGGTTGAGAT800800.12902934325127713No Hit
TAACCTCGGGCCGATCGCAC782000.1260001828452781No Hit
TCGGGGTCCGGTGCGGAGTGCCCTT756310.12186086737559117No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC752450.12123892273904031No Hit
AGGGCCGATCGCAC747870.12050096770396183No Hit
GCGGTCGGCGTC697950.11245758007271338No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTT686120.11055146477468315No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC676060.10893054170636667No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG668080.10764475978935219No Hit
GGTTGGCCTCGGATAGCCGGTCCCC657980.10601739169889526No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC649960.10472516475974038No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT649110.10458820803925636No Hit
CTAGATAACCTCGGGCCGATCGCAC646790.10421439675511182No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC639870.10309940792481856No Hit
AAATAATGGAAT634580.10224705374674756No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA631780.10179590219691792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)