FastQCFastQC Report
Thu 22 Jun 2023
DRR277044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53280058
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC21260283.9902884490103223No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC20934043.929057284434638No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC8770491.6461111960501245No Hit
TCGGTTGGCCTCGGATAGCCGGTC4787700.8985913641460376No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3928760.737379077177431No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT3808610.7148284260501369No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3659940.686924927897038No Hit
TTGGTGACTCTAGATAACCTCG3186000.5979723220271269No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2710880.5087982449268355No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT2497130.4686800453558065No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2410410.4524037867976795No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2320220.43547625267224743No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT2164580.40626457276003713No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2135730.4008497888647193No Hit
CCGGGGTCCGGTGCGGAGTGCCCT2125560.39894100715881353No Hit
CGGTTGGCCTCGGATAGCCGGTC2006070.37651422977054566No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1996500.37471806055466383No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1884290.3536576480453531No Hit
AGATAACCTCGGGCCGATCGCAC1846090.34648798618049553No Hit
AACTGTGGTAATTCTAGAGCT1839750.3452980475359092No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1717670.3223851595657047No Hit
TCGGGCCGATCGCAC1664690.31244147669659067No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1556450.29212618349627173No Hit
TTGGTGACTCTAGATAACCTC1476360.2770942929529093No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1456100.27329174453976757No Hit
ATAACCTCGGGCCGATCGCAC1398970.26256915861465463No Hit
AACCTCGGGCCGATCGCAC1362260.2556791511000232No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1328980.24943291165336196No Hit
TAACTGTGGTAATTCTAGAGCT1256240.23578052411279282No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1214880.2280177698004758No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1202730.225737366877491No Hit
TTTGGTGACTCTAGATAACCTC1182590.22195734096235406No Hit
TACTTGGATAACTGTGGT1117650.20976891579209617No Hit
TTTGGTGACTCTAGATAACCTCG1105890.2075617109876269No Hit
CAGTGGCTGGTTGAGAT1096070.20571861990090176No Hit
ATGCGTGCATTTATCAGA1080400.20277755703644318No Hit
TTTTGGTGACTCTAGATAACCTC1069300.20069422597100026No Hit
ACCTCGGGCCGATCGCAC1040850.1953545170690317No Hit
AGCTAGGAATAATGGAAT1030820.1934720116107982No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC1018670.19119160868781337No Hit
ATTGATCATCGACACTTCGAACGCA995370.186818490325217No Hit
CCTGCGGAAGGATCATTA934580.17540896821095803No Hit
TACTTGGATAACTGTGGTAATTCT925430.17369162773809294No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC871210.16351521238959613No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT867770.1628695674467922No Hit
TTAGATAACCTCGGGCCGATCGCAC852460.15999607207634797No Hit
TAGATAACCTCGGGCCGATCGCAC845550.15869915156623893No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC832220.15619727741287368No Hit
CTAGATAACCTCGGGCCGATCGCAC821130.15411582322226453No Hit
TCGCTTCTGGCGCCAAGCGCCCGG817050.1533500582901017No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA762000.14301786233040512No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC755140.14173032619446474No Hit
GGTTGGCCTCGGATAGCCGGTC737700.1384570564844355No Hit
GTTGGCCTCGGATAGCCGGTCCCC710710.1333913713081919No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC710320.13331817318967634No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT670240.12579565885607707No Hit
ACTGTGGTAATTCTAGAGCT637590.11966766252394095No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA625910.11747547271814156No Hit
GCAGCTAGGAATAATGGAAT609780.11444807361133127No Hit
AGGAATAATGGAAT609240.11434672237030973No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG600480.1127025800159602No Hit
TAACCTCGGGCCGATCGCAC582170.10926602219539627No Hit
TCGCTGCGATCTATTGAAAGTC568240.1066515355520071No Hit
TAGGAATAATGGAAT558960.10490979570630346No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC555320.1042266132668249No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC551860.1035772145743535No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC546710.10261062403498133No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC536730.10073750295091646No Hit
TTTTGGTGACTCTAGATAACCTCG534190.10026077674314844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)