FastQCFastQC Report
Thu 22 Jun 2023
DRR277047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46529766
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC21660584.655209312679544No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC5332041.1459417182540743No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA4860951.044696850613863No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT2765980.5944538814143187No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2734290.587643187373863No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2400740.5159578924166521No Hit
TCGGTTGGCCTCGGATAGCCGGTC2259110.4855193125192162No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT2189180.4704902233980717No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT2069980.44487221362772383No Hit
TTCGGTTGGCCTCGGATAGCCGGTC1891690.4065548062287698No Hit
CGGTTGGCCTCGGATAGCCGGTC1782010.3829827985810202No Hit
CCGGGGTCCGGTGCGGAGTGCCCT1609650.3459398441849031No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTT1558920.3350371458992508No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1442820.31008537631588345No Hit
TCGGGCCGATCGCAC1399770.3008332343644281No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1379510.29647903236822637No Hit
TAGGAATAATGGAAT1272040.2733819894989371No Hit
GGTTGGCCTCGGATAGCCGGTC1269270.2727866716544416No Hit
GTTGGCCTCGGATAGCCGGTC1261560.27112966783456427No Hit
TAAGGCTGCGTTCAGGTCGCAGTC1258430.2704569801619032No Hit
TTAGATAACCTCGGGCCGATCGCAC1248820.26839163558226364No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1244960.2675620590913782No Hit
TCCGGGGTCCGGTGCGGAGTGCCCT1223930.26304237162937805No Hit
GTCCGGTGCGGAGTGCCCTT1098130.23600591500932974No Hit
TTTGGTGACTCTAGATAACCTCG1076190.2312906538150224No Hit
ATAACCTCGGGCCGATCGCAC1059300.2276607193769253No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1047610.22514834912344067No Hit
AGCTAGGAATAATGGAAT1017700.21872020590002536No Hit
AGATAACCTCGGGCCGATCGCAC995840.21402213800086595No Hit
TTCGGGCCGATCGCAC970310.20853532768679733No Hit
ACCTCGGGCCGATCGCAC901220.19368676816470556No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT873800.18779376625276817No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT849260.1825197229661546No Hit
TTAGGAATAATGGAAT847880.18222313862485362No Hit
ACGATGCCGACCGGCGATGCGGCGGCG828850.17813328354155059No Hit
GTTGGCCTCGGATAGCCGGTCCCC774350.16642035122205429No Hit
ATGCGTGCATTTATCAGA762120.16379192622632144No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG754190.1620876408447874No Hit
TTTTGGTGACTCTAGATAACCTC743640.15982027504716012No Hit
TCGGGGTCCGGTGCGGAGTGCCCTT724250.1556530501356916No Hit
TTAACCTCGGGCCGATCGCAC720110.15476329711178863No Hit
TTACCCTACGCCGGCAGGCGCGGGT702060.15088405989404718No Hit
TCTAGATAACCTCGGGCCGATCGCAC689150.14810949188955733No Hit
TCGATGCCGACCGGCGATGCGGCGGCG665170.14295580166897895No Hit
AACGATGCCGACCGGCGATGCGGCGGCG654430.14064760179537547No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA642350.1380514142280449No Hit
TAACTGTGGTAATTCTAGAGCT636100.13670818804461643No Hit
TTTGGTGACTCTAGATAACCTC630010.13539934845148371No Hit
TAACCTCGGGCCGATCGCAC606280.1302993872782425No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC590750.1269617388576594No Hit
CGGGGTCCGGTGCGGAGTGCCCTT574040.12337048933364506No Hit
TGTCCGGTGCGGAGTGCCCTT543510.11680909807283363No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTT542510.11659418188348508No Hit
GTCCGGTGCGGAGTGCCCTTC539120.11586561600159348No Hit
GGTCCGGTGCGGAGTGCCCTT534520.11487700153059012No Hit
TTGGTGACTCTAGATAACCTCG514810.11064100343853008No Hit
GGTTGGCCTCGGATAGCCGGTCCCC510470.10970826717675736No Hit
CTAGATAACCTCGGGCCGATCGCAC502540.10800398179522329No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG498770.10719374776137924No Hit
TGCGGTCGGCGTC497660.10695519079120236No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC494230.10621802826173679No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC492730.10589565397771397No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT491210.10556898136990416No Hit
TCGCTTCTGGCGCCAAGCGCCCGG486630.10458466522268778No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG477660.10265686700423123No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA474120.10189606369393733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)