FastQCFastQC Report
Thu 22 Jun 2023
DRR277048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35443008
Total Bases934.2 Mbp
Sequences flagged as poor quality0
Sequence length10-39
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC13520893.81482576196693No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC13138573.7069568135977624No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC5984061.6883612135854833No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2970730.8381709588531537No Hit
TCGGTTGGCCTCGGATAGCCGGTC2872990.8105942926740304No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2834930.7998559264495836No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT2746540.7749172982157722No Hit
TTGGTGACTCTAGATAACCTCG2133370.6019156161914926No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2038340.5751035578018661No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG1921720.5422000299748825No Hit
AACTGTGGTAATTCTAGAGCT1712050.4830430870878679No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1414090.3989757302766176No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT1397500.39429497631803706No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1308540.36919552651964527No Hit
CGGTTGGCCTCGGATAGCCGGTC1250650.3528622627063707No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT1225870.34587075679355433No Hit
AGATAACCTCGGGCCGATCGCAC1165450.32882367094801884No Hit
CCGGGGTCCGGTGCGGAGTGCCCT1152160.3250739892054309No Hit
TAACTGTGGTAATTCTAGAGCT1151330.32483981043595395No Hit
TTGGTGACTCTAGATAACCTC1114450.3144343730644984No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1102790.3111445845679915No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1060180.299122467257858No Hit
ATAACCTCGGGCCGATCGCAC1026070.2894985662616446No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC997270.2813728451038919No Hit
TCGGGCCGATCGCAC964860.27222858737046246No Hit
AACGATGCCGACCGGCGATGCGGCGGCG862880.24345563446533658No Hit
TTTGGTGACTCTAGATAACCTC820490.23149558863626926No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT818000.23079305232783856No Hit
AGCTAGGAATAATGGAAT801810.22622515560756015No Hit
AACCTCGGGCCGATCGCAC789470.2227435098059397No Hit
ACCTCGGGCCGATCGCAC758210.2139237166326289No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT749810.21155371462828437No Hit
TTTGGTGACTCTAGATAACCTCG733640.20699146076992112No Hit
ATTGATCATCGACACTTCGAACGCA720280.20322202901063027No Hit
TTTTGGTGACTCTAGATAACCTC719140.2029003858814692No Hit
CAGTGGCTGGTTGAGAT714630.20162792051961276No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT636610.17961511618878398No Hit
TTAGATAACCTCGGGCCGATCGCAC629780.17768807884477525No Hit
ACGATGCCGACCGGCGATGCGGCGGCG627450.1770306854316654No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC611000.17238943150649064No Hit
TAGATAACCTCGGGCCGATCGCAC607170.17130882344974782No Hit
AGGAATAATGGAAT596800.16838299954676533No Hit
CTAGATAACCTCGGGCCGATCGCAC588650.1660835333163596No Hit
ACTGTGGTAATTCTAGAGCT570120.16085542175201384No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG566070.15971274221420484No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA529100.14928191196413126No Hit
TAGGAATAATGGAAT519540.14658462396871055No Hit
GGTTGGCCTCGGATAGCCGGTC484300.13664190127429365No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC482710.1361932937520427No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC475570.13417879204834984No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG452640.12770925086268073No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC450850.12720421472127874No Hit
CCTGCGGAAGGATCATTA449030.12669071428700407No Hit
TAACCTCGGGCCGATCGCAC448830.126634285667853No Hit
ATGCGTGCATTTATCAGA440660.12432917657553219No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC401670.1133284172720329No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC398090.11231834498922891No Hit
CAGCTAGGAATAATGGAAT397230.11207570192687934No Hit
GTTGGCCTCGGATAGCCGGTCCCC396050.1117427730738881No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA395700.11164402299037374No Hit
AGCTAGGAATAATGGAATA381450.10762348387586065No Hit
ATAGGAATAATGGAAT381220.10755859096383694No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC379060.10694916187700548No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT375920.10606323255633382No Hit
AAGGAATAATGGAAT362990.10241512232821773No Hit
AGGAATAATGGAATA362680.1023276579685336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)