FastQCFastQC Report
Thu 22 Jun 2023
DRR277051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54647184
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC34165666.252044021152124No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC7818251.4306775624522574No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA4445100.8134179429995881No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTT3392900.6208737123581702No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3263630.5972183305913805No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT3101430.5675370207548115No Hit
TTAGATAACCTCGGGCCGATCGCAC2567130.4697643706581477No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2512370.45974372622750337No Hit
TTCGGTTGGCCTCGGATAGCCGGTC2143970.3923294565370468No Hit
AACGATGCCGACCGGCGATGCGGCGGCG2121770.388267033119218No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA2071500.37906802297443176No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1998460.36570228394568327No Hit
TTTTGGTGACTCTAGATAACCTC1842700.33719944288437625No Hit
ACCGGGGTCCGGTGCGGAGTGCCCT1832450.33532377441443273No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC1708940.3127224268317284No Hit
AGATAACCTCGGGCCGATCGCAC1662770.30427368407491956No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1623300.29705098802529334No Hit
TCGGTTGGCCTCGGATAGCCGGTC1621330.29669049369497247No Hit
ATAACCTCGGGCCGATCGCAC1551060.28383164263322336No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1550320.2836962285192957No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT1528050.27962099565825754No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTT1396860.25561426916343943No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1266200.2317045284529208No Hit
TCTAGATAACCTCGGGCCGATCGCAC1228900.22487892514278504No Hit
TCGATGCCGACCGGCGATGCGGCGGCG1197550.2191421245054457No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC1136060.20788994360624327No Hit
ATGCGTGCATTTATCAGA1135920.20786432471982452No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT1127520.2063271915347001No Hit
AGCTAGGAATAATGGAAT1116750.20435636720091563No Hit
ACCTCGGGCCGATCGCAC1093570.20011461157815563No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCC1070950.19597533150107058No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1067010.19525434284042886No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1061070.1941673700880909No Hit
TTAACCTCGGGCCGATCGCAC1021390.18690624570883654No Hit
CTAGATAACCTCGGGCCGATCGCAC1005670.18402961074810367No Hit
TTCGGGCCGATCGCAC971230.1777273646890936No Hit
CAGTGGCTGGTTGAGAT955030.17476289354635366No Hit
CCGGGGTCCGGTGCGGAGTGCCCTT902810.1652070489121635No Hit
TAGCAGTGGCTGGTTGAGATTT895710.16390780538664168No Hit
TAACCTCGGGCCGATCGCAC875670.1602406447878449No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA838320.15340589187541667No Hit
TCGGGCCGATCGCAC834790.15275992995357274No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTC814880.14911655832073614No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTT791270.14479611611826146No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTA774660.14175661823672378No Hit
CGGTTGGCCTCGGATAGCCGGTCCCC760170.13910506349238416No Hit
TCAGTGGCTGGTTGAGAT758870.13886717383278158No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC755820.13830904809294473No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT754900.13814069541076443No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCT741490.13568677207594082No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT723270.13235265700058763No Hit
TTTTGGTGACTCTAGATAACC705740.12914480643686965No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTT701960.12845309650356365No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC678150.12409605589191934No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC654460.11976097432577679No Hit
ACCTGCGGAAGGATCATTAACGGAGC647270.11844526151612862No Hit
ACTCTAGATAACCTCGGGCCGATCGCAC640170.1171460179906068No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGC627400.114809209565126No Hit
TTTGGTGACTCTAGATAACCTC625380.11443956563251273No Hit
TTTGGTGACTCTAGATAACCTCG618140.11311470322057217No Hit
ACGATGCCGACCGGCGATGCGGCGGCGT602780.11030394539634465No Hit
TCCGGGGTCCGGTGCGGAGTGCCCTTC602380.11023074857800541No Hit
TTTTGGTGACTCTAGATAACCT586500.10732483488993688No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTTC583880.10684539572981472No Hit
TCAGCTAGGAATAATGGAAT571430.10456714475900533No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTA547000.10009664907893515No Hit

[OK]Adapter Content

Adapter graph