FastQCFastQC Report
Thu 22 Jun 2023
DRR277055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49198915
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATT20634774.194151436063173No Hit
TTGGTGACTCTAGATAACCTC20502354.167236208359474No Hit
TTGGTGACTCTAGATAACCT19869674.038639876509472No Hit
AACTGTGGTAATTCTAGAGCT19624633.988833900097187No Hit
TTGGTGACTCTAGATAACCTCG19305353.9239381600183663No Hit
ACTGTGGTAATTCTAGAGCT12380002.5163156545220557No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT8851741.7991738232438663No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC5460521.1098862647682373No Hit
AACGATGCCGACCGGCGATGCGGCGGCG5302101.0776863676770108No Hit
CAGTGGCTGGTTGAGAT5015811.019496060024901No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA4983241.01287599533445No Hit
TTCTAGAGCT3898260.7923467417929847No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2959480.6015335907306086No Hit
TAACTGTGGTAATTCTAGAGCT2924600.5944440034907273No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA2859140.5811388320250559No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2509240.5100193774598485No Hit
GAGGGCGCACCACCGGCCCGTCTCG2363900.4804780755835774No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG2304050.46831317316652205No Hit
CTGTGGTAATTCTAGAGCT2245650.4564429926960788No Hit
ATTGATCATCGACACTTCGAACG2242040.4557092366772723No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT2110760.4290257214005634No Hit
TTGGTGACTCTAGATAACCTCGG2104960.4278468336141153No Hit
ACGATGCCGACCGGCGATGCGGCGGCG2018410.4102549822491004No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2012250.4090029221172865No Hit
ATCGGGGATT2001460.40680978432146314No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1829630.3718842173653626No Hit
ACTGTGGTAATTCTAGAGC1768750.35950996073795527No Hit
AACTGTGGTAATTCTAGAGC1753840.356479406100724No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1716330.348855254226643No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG1666080.33864161435267426No Hit
AACGATGCCGACCGGCGATGCGGCGGC1657510.3368997060199397No Hit
TAGGAATAATGGAAT1588390.32285061570971635No Hit
AGCAGTGGCTGGTTGAGATTT1532060.3114011762251261No Hit
ATTGATCATCGACACTTCGAACGCA1530040.31099059806501833No Hit
AACTGTGGTAATTCTAGAGCTA1515090.3079519131671908No Hit
TTTGGTGACTCTAGATAACCTC1511510.3072242548438314No Hit
TTGGTGACTCTAGATAACC1493330.30352905140286124No Hit
TTTGGTGACTCTAGATAACCTCG1373690.2792114419596448No Hit
AGGAATAATGGAAT1346980.27378246044653626No Hit
AGAGCAGTGGCTGGTTGAGATTTAA1214310.2468164186141097No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG1134550.23060467898529877No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT1098700.22331793292595983No Hit
AGCTAGGAATAATGGAAT1095790.22272645646758674No Hit
TGGTGACTCTAGATAACCT1089250.22139715886010902No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1047880.2129884368384953No Hit
TGGTGACTCTAGATAACCTC1039880.2113623847192565No Hit
GGTTGGCCTCGGATAGCCGGTC999580.20317114716859103No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG963900.19591895471678591No Hit
TGGTGACTCTAGATAACCTCG930510.18913221968411295No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC907600.18447561292764283No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT867900.17640632928592023No Hit
ACGATGCCGACCGGCGATGCGGCGGC836930.17011147501931698No Hit
TTTGGTGACTCTAGATAACCT809060.1644467159489188No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG790060.1605848421657266No Hit
ATGGGGATCGGGGATT789510.16047305108252896No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC749070.1522533576197768No Hit
ACAAGCATCAAGCACGCAGC732140.14881222482243767No Hit
TTGATCATCGACACTTCGAACG728290.14802968724005397No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA716960.14572679092618201No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT708600.14402756646157744No Hit
TTGGTGACTCTAGATA707160.14373487708011445No Hit
CCGGGGTCCGGTGCGGAGTGCCCT687040.13964535600022887No Hit
CCTGCGGAAGGATCATTA672530.13669610396895948No Hit
TTGGTGACTCTAGATAA669070.13599283642738869No Hit
GTTGGCCTCGGATAGCCGGTC657570.1336553865059829No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC647930.13169599370230015No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG642280.13054759439308775No Hit
AGTGGCTGGTTGAGAT635410.1291512221356914No Hit
TCGGGCCGATCGCAC625140.1270637777276186No Hit
ATAACCTCGGGCCGATCGCAC583080.11851480871072056No Hit
AAAGATTAAGCCATGCATGTCTA575370.11694770098080415No Hit
ATAACCTCGGGCCGATCGCA563980.11463260927603791No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC561590.1141468262054153No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT560580.1139415371253614No Hit
ATTGATCATCGACACTTCGAACGC559860.1137951924346299No Hit
AACGATGCCGACCGGCGATGCGGCGG552570.11231345244097354No Hit
TTGATCATCGACACTTCGAACGCA549420.11167319441902326No Hit
TCGGGCCGATCGCA546380.11105529461371251No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCT525280.10676658214922018No Hit
TTTCTAGAGCT523640.1064332414647762No Hit
AGGGCGCACCACCGGCCCGTCTCG520380.1057706252261864No Hit
ATTGATCATCGACACTTCGAAC513580.10438848092483341No Hit
CGAGGGCGCACCACCGGCCCGTCTCG512540.10417709414933236No Hit
TAACTGTGGTAATTCTAGAGCTA504980.10264047489665169No Hit
CGGTTGGCCTCGGATAGCCGGTC503770.10239453451361683No Hit
TTGGTGACTCTAGATAAC503460.10233152499399631No Hit
TTTGGTGACTCTAGATAACCTCGG500720.10177460214315702No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCG494500.10051034662044885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)