FastQCFastQC Report
Thu 22 Jun 2023
DRR277056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34698419
Total Bases847.4 Mbp
Sequences flagged as poor quality0
Sequence length10-36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTGTGGTAATTCTAGAGCT17027104.907168825184802No Hit
TTGGTGACTCTAGATAACCTCG16218704.674189910497075No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT15294544.407849245235064No Hit
TTGGTGACTCTAGATAACCTC11380113.2797200356592615No Hit
TTGGTGACTCTAGATAACCT11165333.2178209618138514No Hit
ACTGTGGTAATTCTAGAGCT9160242.639958898415516No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT7397022.1318031810037223No Hit
GAGGGCGCACCACCGGCCCGTCTCG3782611.0901390060452034No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG3407120.9819237009040671No Hit
AACGATGCCGACCGGCGATGCGGCGGCG2984560.8601429361954503No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2734670.7881252457064398No Hit
TAACTGTGGTAATTCTAGAGCT2686410.7742168310319845No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2398110.6911294719220492No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2379250.6856940657728526No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1985380.5721816893155852No Hit
CTGTGGTAATTCTAGAGCT1900970.5478549325258882No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT1804310.5199977555173335No Hit
TTGGTGACTCTAGATAACCTCGG1720190.49575457602261364No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1700730.49014625133208517No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1671100.4816069573659825No Hit
ATTGATCATCGACACTTCGAACG1597890.4605080133478128No Hit
AACTGTGGTAATTCTAGAGCTA1562620.45034328509319116No Hit
TTTGGTGACTCTAGATAACCTCG1523570.4390891700281791No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG1500660.4324865637249928No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1490200.4294720171544415No Hit
ATTGATCATCGACACTTCGAACGCA1437020.4141456704410653No Hit
AACTGTGGTAATTCTAGAGC1412880.4071885811281488No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1389520.40045628591896365No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1353800.39016186875834313No Hit
AACGATGCCGACCGGCGATGCGGCGGC1339440.38602335166913515No Hit
CAGTGGCTGGTTGAGAT1326100.3821787961001912No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1321780.38093378260260213No Hit
TTCTAGAGCT1295690.37341470803035726No Hit
ACTGTGGTAATTCTAGAGC1178980.3397791697656311No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT1128220.3251502611689599No Hit
GGTTGGCCTCGGATAGCCGGTC1049140.30235959742142715No Hit
TTTGGTGACTCTAGATAACCTC1021420.2943707608118975No Hit
TGGTGACTCTAGATAACCTCG791730.22817466121439137No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG790490.2278172962289723No Hit
TTTCTAGAGCT786390.22663568619653826No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG777850.2241744789582488No Hit
TTGGTGACTCTAGATAACC765140.22051148785770325No Hit
CACTCGCCGAATCCCGGGGCCGAGGG732070.21098079425463162No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT672070.1936889401214505No Hit
ACGATGCCGACCGGCGATGCGGCGGC665330.19174648850715648No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC658260.18970893169512998No Hit
CCGGGGTCCGGTGCGGAGTGCCCT647930.18673185080853397No Hit
CGAGGGCGCACCACCGGCCCGTCTCG646790.1864033055800035No Hit
AGCTAGGAATAATGGAAT642480.18516117405810334No Hit
AGAGCAGTGGCTGGTTGAGATTTAA635650.18319278466260955No Hit
TTTGGTGACTCTAGATAACCT631340.18195065314070938No Hit
TGGTGACTCTAGATAACCT615760.17746053501746No Hit
TTGATCATCGACACTTCGAACG599380.1727398588391016No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA591790.17055243929125416No Hit
TGGTGACTCTAGATAACCTC590610.17021236615996826No Hit
AGGGCGCACCACCGGCCCGTCTCG581720.1676502897725686No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG577600.16646291578875683No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT566960.16339649365580605No Hit
TTGATCATCGACACTTCGAACGCA565560.16299301705936514No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA528210.1522288378614599No Hit
ATTGATCATCGACACTTCGAACGC524850.15126049403000177No Hit
TAACTGTGGTAATTCTAGAGCTA500940.1443696901579291No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGC500040.14411031234593139No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG496690.1431448504901621No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG494290.14245317632483487No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC485830.1400150248920563No Hit
AGCAGTGGCTGGTTGAGATTT455090.13115583162448988No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCG447430.1289482382468204No Hit
TTGGTGACTCTAGATAACCTCGGGCCG439940.12678963845586164No Hit
ATGGGGATCGGGGATT437480.1260806724364012No Hit
AACGATGCCGACCGGCGATGCGGCGG436750.12587028821111418No Hit
TTTGGTGACTCTAGATAACCTCGG428480.12348689431642404No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT425280.12256466209598772No Hit
ATCGGGGATT418450.12059627270049394No Hit
GTTGGCCTCGGATAGCCGGTC412970.11901695002299673No Hit
CGGTTGGCCTCGGATAGCCGGTC411580.11861635540224469No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC410800.11839156129851333No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC408810.1178180481364295No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC401340.11566521229684845No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC389650.112296182716567No Hit
CCTGCGGAAGGATCATTA383690.11057852520600434No Hit
AACCCCATTCGTGATGGGGATCGGGGATT377590.10882052003579759No Hit
TAGGAATAATGGAAT376750.10857843407793305No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT372630.1073910600941213No Hit
TCGGTTGGCCTCGGATAGCCGGTC370970.10691265212976996No Hit
AGGGCGCACCACCGGCCCGTCTCGC367790.10599618386071136No Hit
ATTGATCATCGACACTTCGAAC362120.10436210364512574No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCC361920.10430446413134846No Hit
GTTGAACCCCATTCGTGATGGGGATCGGG361330.10413442756570551No Hit
TCGCTTCTGGCGCCAAGCGCCCGG352630.10162710871639426No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC348820.10052907597893725No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGG347620.10018323889627365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)