FastQCFastQC Report
Thu 22 Jun 2023
DRR277059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28183206
Total Bases660.9 Mbp
Sequences flagged as poor quality0
Sequence length10-39
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCG13948064.949067895256487No Hit
AACTGTGGTAATTCTAGAGCT13158924.669064264725596No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT11068533.927349500266222No Hit
TTGGTGACTCTAGATAACCTC10114713.588913908517008No Hit
TTGGTGACTCTAGATAACCT8798373.1218485221305197No Hit
ACTGTGGTAATTCTAGAGCT7912982.80769334759147No Hit
CAGTGGCTGGTTGAGAT6081822.1579588922566155No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT4212751.4947731638480022No Hit
AACGATGCCGACCGGCGATGCGGCGGCG3107141.1024792566182855No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2651500.9408085084429357No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2275820.8075092663340006No Hit
TTCTAGAGCT2074410.736044721100928No Hit
TAACTGTGGTAATTCTAGAGCT1907950.6769811780817271No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG1825450.647708426074734No Hit
AGCAGTGGCTGGTTGAGATTT1775800.6300915516850709No Hit
GAGGGCGCACCACCGGCCCGTCTCG1689360.5994208040064711No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1620210.5748849155060641No Hit
ATTGATCATCGACACTTCGAACG1463040.519117661773469No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1460070.5180638427012172No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1456990.516970993292956No Hit
CTGTGGTAATTCTAGAGCT1389750.49311281335416557No Hit
AGAGCAGTGGCTGGTTGAGATTTAA1362820.4835574774566102No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1300820.4615585607968093No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1199710.42568258557951144No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1198870.4253845357408948No Hit
TTGGTGACTCTAGATAACCTCGG1158580.41108878812438865No Hit
GTTGGCCTCGGATAGCCGGTC1047170.3715581541716723No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1032740.36643808372972186No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT979700.3476183653484987No Hit
AACTGTGGTAATTCTAGAGC943840.334894475809459No Hit
TTTGGTGACTCTAGATAACCTCG927700.3291676610531818No Hit
ACTGTGGTAATTCTAGAGC917120.3254136523715577No Hit
AACTGTGGTAATTCTAGAGCTA910210.32296183762769926No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG901090.3197258679512899No Hit
AACGATGCCGACCGGCGATGCGGCGGC891590.31635506620503007No Hit
GGTTGGCCTCGGATAGCCGGTC814980.28917221128071807No Hit
ATTGATCATCGACACTTCGAACGCA800260.28394924268019756No Hit
AGTGGCTGGTTGAGAT720210.25554580270250304No Hit
TGGTGACTCTAGATAACCTCG716550.25424715697710193No Hit
ATCGGGGATT691660.2454156564019012No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG673590.23900403665927855No Hit
TTTGGTGACTCTAGATAACCTC673290.23889759028834406No Hit
TTGGTGACTCTAGATAACC611460.21695899323873943No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG538810.19118123041076307No Hit
TGGTGACTCTAGATAACCTC536010.19018773094870756No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA521090.1848937981008974No Hit
CCGGGGTCCGGTGCGGAGTGCCCT512780.18194523362601117No Hit
TGGTGACTCTAGATAACCT511200.1813846160724227No Hit
ACGATGCCGACCGGCGATGCGGCGGC507620.18011435604593742No Hit
AGCTAGGAATAATGGAAT500930.17774060197409763No Hit
TTGATCATCGACACTTCGAACG487270.17289374388421247No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG468320.16616988145351524No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT467490.16587537982726308No Hit
TAGGAATAATGGAAT464560.16483575360446925No Hit
ACAAGCATCAAGCACGCAGC445260.15798770374101512No Hit
CCTGCGGAAGGATCATTA444120.15758320753146396No Hit
CAGTGGCTGGTTGAGA438400.1555536300589791No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG424150.15049742743958938No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT413030.14655181529028316No Hit
ATGGTCGCACGACCGTCACG407180.14447611105706No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC381790.13546719986363512No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC380730.1350910893529998No Hit
AGGAATAATGGAAT380310.13494206443369147No Hit
TACCCTACGCCGGCAGGCGCGGGTA378610.1343388683317292No Hit
TTTCTAGAGCT375170.13311828327834668No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC363420.12894913375007797No Hit
ATGGGGATCGGGGATT359440.12753694522901335No Hit
AGGGCGCACCACCGGCCCGTCTCG356900.12663569928843438No Hit
TTTGGTGACTCTAGATAACCT354270.12570251943657512No Hit
ATGTACACTAGGGATAAC336870.11952862992237291No Hit
CGGTTGGCCTCGGATAGCCGGTC335840.11916316404883107No Hit
TACCCTACGCCGGCAGGCGCGGGT330770.11736422038003766No Hit
GTGGCTGGTTGAGAT330380.11722584009782279No Hit
AACGATGCCGACCGGCGATGCGGCGG315760.11203835362094718No Hit
GTCCGGTGCGGAGTGCCCTT311920.11067584007298531No Hit
GGTGACTCTAGATAACCTCG300040.10646056378397831No Hit
TCGGTTGGCCTCGGATAGCCGGTC298130.10578285522236186No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT292240.10369295813968077No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT288840.10248656593575621No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT282770.10033280103051441No Hit
TAACTGTGGTAATTCTAGAGCTA282320.10017313147411262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)