FastQCFastQC Report
Thu 22 Jun 2023
DRR277060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59124643
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCG28929934.893040960940771No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT25533114.318522481395786No Hit
AACTGTGGTAATTCTAGAGCT23593753.9905103528489807No Hit
TTGGTGACTCTAGATAACCTC21324353.6066771684355037No Hit
TTGGTGACTCTAGATAACCT18245303.0859044679559418No Hit
ACTGTGGTAATTCTAGAGCT13999182.3677403007744164No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT11116481.8801771031412402No Hit
GAGGGCGCACCACCGGCCCGTCTCG10224121.7292484962657617No Hit
GAGGGCGCACCACCGGCCCGTCTCGC6877541.1632273196135832No Hit
AACGATGCCGACCGGCGATGCGGCGGCG5916821.0007366978943113No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG5454270.9225036673794377No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA4086980.691248148424338No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC3721510.6294346673687315No Hit
TAACTGTGGTAATTCTAGAGCT3699550.6257204800374017No Hit
ATTGATCATCGACACTTCGAACG3641760.6159462138316844No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3561610.602390106609185No Hit
ATTGATCATCGACACTTCGAACGCA3460250.5852466627155787No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA3283110.5552862281130391No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG3135440.5303101787861958No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT3102260.5246983055779296No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA3055010.5167067139838798No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2816390.4763479079273257No Hit
TTGGTGACTCTAGATAACCTCGG2803570.4741796073085803No Hit
ACGATGCCGACCGGCGATGCGGCGGCG2739620.46336347434689795No Hit
CTGTGGTAATTCTAGAGCT2640010.44651601532714535No Hit
TTCTAGAGCT2453600.4149877065642494No Hit
TTTGGTGACTCTAGATAACCTCG2439660.41262997562623766No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG2278850.3854315027322871No Hit
GGTTGGCCTCGGATAGCCGGTC2201830.37240478559845175No Hit
AACTGTGGTAATTCTAGAGCTA2185100.36957517020441033No Hit
AACGATGCCGACCGGCGATGCGGCGGC2005880.339262936437519No Hit
AACTGTGGTAATTCTAGAGC1818030.30749107440699475No Hit
ACTGTGGTAATTCTAGAGC1788760.3025405159740246No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT1733160.29313665369615843No Hit
TTTGGTGACTCTAGATAACCTC1694190.2865454933909707No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1684140.2848456945439823No Hit
CAGTGGCTGGTTGAGAT1660020.2807661773112102No Hit
AGGGCGCACCACCGGCCCGTCTCG1603160.27114920592416936No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG1563980.26452252743411914No Hit
CGAGGGCGCACCACCGGCCCGTCTCG1550250.2622003146809698No Hit
TGGTGACTCTAGATAACCTCG1508520.25514234394616137No Hit
TAGGAATAATGGAAT1460790.24706956792956872No Hit
CACTCGCCGAATCCCGGGGCCGAGGG1371340.23194051252030395No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1338300.22635231810194612No Hit
TTGGTGACTCTAGATAACC1282070.21684190126949268No Hit
TTGATCATCGACACTTCGAACGCA1245100.21058900939156622No Hit
TTGATCATCGACACTTCGAACG1215710.2056181548529604No Hit
GTTGGCCTCGGATAGCCGGTC1214520.20541688513873987No Hit
TGGTGACTCTAGATAACCTC1170210.19792254813276422No Hit
TTTCTAGAGCT1145530.1937483157403589No Hit
AGGAATAATGGAAT1140720.1929347801727953No Hit
ATTGATCATCGACACTTCGAACGC1090690.1844729954648521No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG1086180.18371020016137773No Hit
TGGTGACTCTAGATAACCT1076440.18206283292061484No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT1039030.1757355219886909No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1032400.17461416215231945No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG1027200.1737346642414399No Hit
ACGATGCCGACCGGCGATGCGGCGGC1020500.1726014650101143No Hit
AGCTAGGAATAATGGAAT1018350.17223782645080832No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA1018310.1722310610822631No Hit
TTTGGTGACTCTAGATAACCT997740.16875197030787992No Hit
CCGGGGTCCGGTGCGGAGTGCCCT995630.16839509711711917No Hit
AGGGCGCACCACCGGCCCGTCTCGC949870.16065551550137902No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG897820.15185207968190184No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC887630.15012860204500517No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCG865180.14633153894899628No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA860950.14561610122533847No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG841570.1423382801651758No Hit
TTGGTGACTCTAGATAACCTCGGGCCG832420.14079070211045502No Hit
CGGTTGGCCTCGGATAGCCGGTC828130.1400651163339794No Hit
ATTGATCATCGACACTTCGAAC809390.13689554117054034No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC808860.13680590003731607No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG806300.13637291645042154No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT775060.13108916361659892No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC774760.13103842335250973No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC770960.13039571334071312No Hit
TCGCTTCTGGCGCCAAGCGCCCGG766000.12955680764110492No Hit
TCGGTTGGCCTCGGATAGCCGGTC760280.1285893599391374No Hit
AGAGCAGTGGCTGGTTGAGATTTAA759350.12843206512046085No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC739750.12511703453329942No Hit
TAACTGTGGTAATTCTAGAGCTA736670.12459610115531691No Hit
CGCTTCTGGCGCCAAGCGCCCGG730690.1235846785578054No Hit
TACCCTACGCCGGCAGGCGCGGGTA725120.1226426009878825No Hit
GAGGGCGCACCACCGGCCCGTCTC717330.12132504546369946No Hit
ACCGGCCCGTCTCG706970.11957281501048557No Hit
AACGATGCCGACCGGCGATGCGGCGG661000.11179771520988296No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC652640.11038375318393043No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC649270.10981377088399502No Hit
ATGGGGATCGGGGATT642280.10863152273071652No Hit
TTTGGTGACTCTAGATAACCTCGG625690.10582558612658347No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGG609560.1030974512607205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)