FastQCFastQC Report
Thu 22 Jun 2023
DRR277063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28438281
Total Bases670.9 Mbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATT13819904.859611591853952No Hit
AACTGTGGTAATTCTAGAGCT11725504.123139510436654No Hit
TTGGTGACTCTAGATAACCTCG10167743.575370817947822No Hit
TTGGTGACTCTAGATAACCT9718073.4172494462657568No Hit
TTGGTGACTCTAGATAACCTC9512993.3451353828313324No Hit
ACTGTGGTAATTCTAGAGCT7511592.6413656999872814No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT5613891.9740609497458725No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG3023261.063095199038226No Hit
GTTGGCCTCGGATAGCCGGTC2654480.9334178813409995No Hit
TTCTAGAGCT2626680.9236423256384589No Hit
CAGTGGCTGGTTGAGAT2574820.9054063429501945No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2390410.8405606513276944No Hit
GAGGGCGCACCACCGGCCCGTCTCG2258030.794010720971496No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2188230.769466340106844No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1714500.6028845414390553No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1621690.5702489542177321No Hit
TAACTGTGGTAATTCTAGAGCT1601500.5631493689790884No Hit
ATCGGGGATT1513420.5321770327819745No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1412790.4967916309709437No Hit
ATTGATCATCGACACTTCGAACG1404900.49401720167263274No Hit
CTGTGGTAATTCTAGAGCT1366140.4803876858801698No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1281190.4505159787963274No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT1249160.4392529914167456No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1191730.4190583812010297No Hit
ATTGATCATCGACACTTCGAACGCA1171390.4119060501582356No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1142410.40171556079637866No Hit
GGTTGGCCTCGGATAGCCGGTC1018310.35807719882928224No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG957210.33659207460535323No Hit
ACTGTGGTAATTCTAGAGC955290.3359169283122282No Hit
AACTGTGGTAATTCTAGAGC949490.3338774238850794No Hit
TTGGTGACTCTAGATAACCTCGG946410.33279437670652456No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA945560.332495483816339No Hit
AACTGTGGTAATTCTAGAGCTA810470.28499261259849007No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA801900.2819790689880306No Hit
AGCAGTGGCTGGTTGAGATTT751230.26416153634602596No Hit
TTGGTGACTCTAGATAACC722660.25411521884884675No Hit
GTCCGGTGCGGAGTGCCCTT713060.2507394873832212No Hit
AACGATGCCGACCGGCGATGCGGCGGC704500.24772946015970515No Hit
TAGGAATAATGGAAT679290.23886464867549484No Hit
TTTGGTGACTCTAGATAACCTCG674150.23705722578660784No Hit
TTTGGTGACTCTAGATAACCTC673690.23689547198721328No Hit
ACCGGCTCCGGGACGGCTGGG671180.23601285886442996No Hit
ACGATGCCGACCGGCGATGCGGCGGCG662240.23286920893706622No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG646790.22743639110957514No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT638530.2245318554943599No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG557930.19618977673087906No Hit
AGGAATAATGGAAT545350.1917661619561323No Hit
TGGTGACTCTAGATAACCT536270.1885732826115615No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC521640.18342880851342597No Hit
TCGCTTCTGGCGCCAAGCGC515960.18143150072959754No Hit
GTCCGGTGCGGAGTGCCCT513640.18061569895873805No Hit
TTGATCATCGACACTTCGAACG510980.1796803400318043No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT505300.17768303224797588No Hit
TGGTGACTCTAGATAACCTCG491510.17283393465308258No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG487540.1714379290365687No Hit
TGGTGACTCTAGATAACCTC485040.1705588323007287No Hit
CCGGGGTCCGGTGCGGAGTGCCCT477850.16803055008845297No Hit
ATGGGGATCGGGGATT477700.16797780428430256No Hit
AGAGCAGTGGCTGGTTGAGATTTAA477430.16788286183683185No Hit
TACCCTACGCCGGCAGGCGCGGGT466680.16410274587271995No Hit
AGCTAGGAATAATGGAAT466450.16402186897302268No Hit
AGGGCGCACCACCGGCCCGTCTCG442700.1556704499825429No Hit
TTGATCATCGACACTTCGAACGCA441990.15542078650956434No Hit
ACCCTACGCCGGCAGGCGCGGGT435520.15314568415721047No Hit
TACCCTACGCCGGCAGGCGCGGGTA416080.14630982793931885No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG398130.13999791337598783No Hit
CGGTTGGCCTCGGATAGCCGGTC387000.13608417470802825No Hit
TCGGTTGGCCTCGGATAGCCGGTC380780.13389698202925837No Hit
TTTGGTGACTCTAGATAACCT369950.1300887349695996No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC362590.1275006741792867No Hit
CGAGGGCGCACCACCGGCCCGTCTCG362290.12739518257098592No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC360800.1268712409164253No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC359690.12648092196571234No Hit
ACAAGCATCAAGCACGCAGC356350.12530644872663013No Hit
ACGATGCCGACCGGCGATGCGGCGGC346740.12192720087406125No Hit
AGTGGCTGGTTGAGAT345100.12135051341535025No Hit
ATGGTCGCACGACCGTCACG339570.11940595143567223No Hit
ATTCTAGAGCT335940.11812950297523257No Hit
TTTCTAGAGCT335620.11801697859304505No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC334570.11764775796399227No Hit
GATCGGGGATT316850.11141672030035851No Hit
ATTGATCATCGACACTTCGAACGC312030.10972182179365905No Hit
CACTCGCCGAATCCCGGGGCCGAGGG311300.10946512554679377No Hit
TCGCTTCTGGCGCCAAGCGCCCGG302920.10651839328825817No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG302140.1062441151066761No Hit
CGGCCGAGGTGGGATCCCG299880.10544941165747676No Hit
GAGGGCGCACCACCGGCCCGTCTC296590.10429252035311135No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC296260.10417647958398048No Hit
TTGGTGACTCTAGATA295780.1040076930106992No Hit
CCTGCGGAAGGATCATTA294950.10371583289440034No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA292590.10288596557576739No Hit
AACGATGCCGACCGGCGATGCGGCGG287630.10114183765186088No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT286060.10058976490175339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)