FastQCFastQC Report
Thu 22 Jun 2023
DRR277064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101304975
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATT29721152.93382926159352No Hit
TTGGTGACTCTAGATAACCTCG20535732.0271195960514277No Hit
AACTGTGGTAATTCTAGAGCT19559731.930776844868675No Hit
TTGGTGACTCTAGATAACCTC18623001.8383105074553345No Hit
TTGGTGACTCTAGATAACCT15149941.495478380997577No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT14752701.4562660915715147No Hit
ACTGTGGTAATTCTAGAGCT12657431.2494381445728604No Hit
AACGATGCCGACCGGCGATGCGGCGGCG7029900.6939343304709369No Hit
GAGGGCGCACCACCGGCCCGTCTCG6794630.6707103969967911No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG6552010.6467609315337178No Hit
GTTGGCCTCGGATAGCCGGTC6497310.6413613941467337No Hit
GAGGGCGCACCACCGGCCCGTCTCGC5329350.5260699190735697No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA4381710.4325266355378894No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4067310.40149163454213377No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC3915960.3865515982803411No Hit
ATTGATCATCGACACTTCGAACG3806600.37575647197978185No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT3604010.3557584412808946No Hit
ATTGATCATCGACACTTCGAACGCA3593130.35468445651361147No Hit
GGTTGGCCTCGGATAGCCGGTC3023490.29845424669420234No Hit
AACGATGCCGACCGGCGATGCGGCGGC2958180.2920073767354466No Hit
TAACTGTGGTAATTCTAGAGCT2934630.28968271301582177No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA2766970.2731326867214567No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG2764530.2728918298434998No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA2724460.2689364466059046No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2509660.24773314439888072No Hit
TTGGTGACTCTAGATAACCTCGG2474950.24430685659810886No Hit
ACGATGCCGACCGGCGATGCGGCGGCG2460870.24291699395809535No Hit
GTCCGGTGCGGAGTGCCCTT2453790.24221811416467948No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG2452520.2420927501339396No Hit
CTGTGGTAATTCTAGAGCT2364710.2334248638825487No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT2326940.22969651786597847No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG2221970.21933473652207108No Hit
TTCTAGAGCT2131310.21038552154027976No Hit
GTCCGGTGCGGAGTGCCCT1961490.19362227768182166No Hit
AACTGTGGTAATTCTAGAGCTA1844360.18206016042153902No Hit
TTTGGTGACTCTAGATAACCTCG1836670.18130106640863392No Hit
CAGTGGCTGGTTGAGAT1740230.17178129701922337No Hit
TTTGGTGACTCTAGATAACCTC1631470.16104539781980104No Hit
CCGGGGTCCGGTGCGGAGTGCCCT1605860.15851738771960605No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1431540.14130994060262095No Hit
TTGATCATCGACACTTCGAACGCA1372840.13551555587472383No Hit
TAGGAATAATGGAAT1370440.13527864747017607No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1366870.1349262462184113No Hit
AACTGTGGTAATTCTAGAGC1356640.1339164241440265No Hit
CACTCGCCGAATCCCGGGGCCGAGGG1340610.13233407342531797No Hit
TTGATCATCGACACTTCGAACG1332060.1314900872341166No Hit
ATTGATCATCGACACTTCGAACGC1311490.1294595847834719No Hit
ACTGTGGTAATTCTAGAGC1286740.1270164668615732No Hit
ACCGGCTCCGGGACGGCTGGG1271650.1255269052679792No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT1252920.12367803259415444No Hit
AACGATGCCGACCGGCGATGCGGCGG1247140.12310747818653527No Hit
TTGGTGACTCTAGATAACC1226510.12107105302577688No Hit
AGGGCGCACCACCGGCCCGTCTCG1138200.11235381085677185No Hit
CGAGGGCGCACCACCGGCCCGTCTCG1123840.11093630890289445No Hit
ACGATGCCGACCGGCGATGCGGCGGC1080900.10669762269819424No Hit
TACCCTACGCCGGCAGGCGCGGGT1079010.10651105732961288No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1053050.10394849808708802No Hit
TCGCTTCTGGCGCCAAGCGC1042230.10288043602991859No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA1041500.10280837639020197No Hit
CGGTTGGCCTCGGATAGCCGGTC1030320.10170477807235036No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG1020530.10073838920546596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)