FastQCFastQC Report
Thu 22 Jun 2023
DRR277067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40995418
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length10-36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATT21014575.1260777484937465No Hit
AACTGTGGTAATTCTAGAGCT19157464.673073463966144No Hit
TTGGTGACTCTAGATAACCTCG17028144.153669075895262No Hit
TTGGTGACTCTAGATAACCTC12281432.9958055312425405No Hit
TTGGTGACTCTAGATAACCT12078182.946226819787519No Hit
ACTGTGGTAATTCTAGAGCT11654602.842903077607356No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT10396282.535961457936592No Hit
GAGGGCGCACCACCGGCCCGTCTCG4690131.1440620022462022No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG3480270.8489412158207534No Hit
GAGGGCGCACCACCGGCCCGTCTCGC3024840.7378483127065566No Hit
AACGATGCCGACCGGCGATGCGGCGGCG2782250.6786734068670797No Hit
CAGTGGCTGGTTGAGAT2759710.6731752314368401No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2703740.6595224861471104No Hit
TAACTGTGGTAATTCTAGAGCT2681080.6539950391529121No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2552500.6226305583711819No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT2412660.5885194291713284No Hit
ATTGATCATCGACACTTCGAACG2406060.5869094931536007No Hit
CTGTGGTAATTCTAGAGCT2232650.5446096439363053No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1913540.46676923747917387No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1879950.4585756388677388No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG1868910.45588265498353986No Hit
AACTGTGGTAATTCTAGAGCTA1817550.44335442560922295No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG1807450.44089073564270037No Hit
TTCTAGAGCT1780310.4342704835940446No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1762070.4298212058723246No Hit
TTGGTGACTCTAGATAACCTCGG1681060.4100604609032161No Hit
ATTGATCATCGACACTTCGAACGCA1652560.40310846446302856No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT1513630.3692193112898617No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1467260.35790829111682676No Hit
TTTGGTGACTCTAGATAACCTCG1464380.3572057735818183No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1366620.3333592061434768No Hit
AACTGTGGTAATTCTAGAGC1347860.32878308497793585No Hit
AACGATGCCGACCGGCGATGCGGCGGC1305000.3183282580506924No Hit
ACTGTGGTAATTCTAGAGC1271710.31020783834915405No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1196480.2918570070440555No Hit
AGAGCAGTGGCTGGTTGAGATTTAA1126350.27475021720720105No Hit
AGCAGTGGCTGGTTGAGATTT1089450.2657492112899056No Hit
GGTTGGCCTCGGATAGCCGGTC1064890.25975829786636156No Hit
TTTGGTGACTCTAGATAACCTC1058380.2581703155216029No Hit
GTTGGCCTCGGATAGCCGGTC1035710.25264042923040814No Hit
AGGGCGCACCACCGGCCCGTCTCG926310.2259545200880742No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG898950.2192806035054942No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG891870.21755358123193183No Hit
TTTCTAGAGCT849570.20723535493649559No Hit
TTGATCATCGACACTTCGAACG842590.2055327256328988No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT842520.20551565055392287No Hit
TGGTGACTCTAGATAACCTCG790180.19274837007394338No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC769600.187728296855029No Hit
CACTCGCCGAATCCCGGGGCCGAGGG767820.18729410198964186No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG766090.1868721036092375No Hit
TTGGTGACTCTAGATAACC755120.18419619480401445No Hit
CCGGGGTCCGGTGCGGAGTGCCCT696590.16991898948316614No Hit
TTGATCATCGACACTTCGAACGCA677740.16532091464465615No Hit
CGAGGGCGCACCACCGGCCCGTCTCG674430.16451350733879577No Hit
TTTGGTGACTCTAGATAACCT664230.16202542440230758No Hit
ACGATGCCGACCGGCGATGCGGCGGC663610.16187418798852107No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA656930.16024473759482094No Hit
ATCGGGGATT647940.1580518095949162No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA643360.15693461157049307No Hit
TGGTGACTCTAGATAACCT643110.15687362914557915No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG639510.1559954822268186No Hit
AGCTAGGAATAATGGAAT633180.15445140722799802No Hit
TGGTGACTCTAGATAACCTC622900.15194380991553738No Hit
ATTGATCATCGACACTTCGAACGC586090.14296475767121095No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG580400.14157679768017No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC563590.13747633942895765No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC528940.12902417533588753No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT528530.1289241641590287No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC527350.12863632711343498No Hit
TAACTGTGGTAATTCTAGAGCTA517730.1262897234027471No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGC502600.12259906704695633No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC488900.11925723016167318No Hit
AACGATGCCGACCGGCGATGCGGCGG482230.11763021906496965No Hit
ATGGGGATCGGGGATT473320.11545680544103734No Hit
AGGGCGCACCACCGGCCCGTCTCGC472450.11524458660233687No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC462690.11286383273369721No Hit
GAGGGCGCACCACCGGCCCGTCTC451810.11020987760144317No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT447100.1090609687160648No Hit
TCGCTTCTGGCGCCAAGCGCCCGG443550.10819501828228707No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT443030.10806817483846609No Hit
ATTGATCATCGACACTTCGAAC433150.10565814940586775No Hit
ACAAGCATCAAGCACGCAGC427600.1043043395727786No Hit
TACCCTACGCCGGCAGGCGCGGGTA422810.10313591631142778No Hit
CGGTTGGCCTCGGATAGCCGGTC420820.10265049620911293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)