FastQCFastQC Report
Thu 22 Jun 2023
DRR277068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38186961
Total Bases885.6 Mbp
Sequences flagged as poor quality0
Sequence length10-39
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCT16898824.4252853742406995No Hit
TTGGTGACTCTAGATAACCTC16226054.2491074374837No Hit
AACTGTGGTAATTCTAGAGCT16110504.218848417919404No Hit
TTGGTGACTCTAGATAACCTCG15154723.968558796810252No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT14426453.777847103360752No Hit
ACTGTGGTAATTCTAGAGCT12648943.3123714662709087No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT11784443.0859852922048443No Hit
TTCTAGAGCT3449690.9033685608027305No Hit
GAGGGCGCACCACCGGCCCGTCTCG3109940.8143984015905323No Hit
GTTGGCCTCGGATAGCCGGTC3047330.7980027528244523No Hit
CTGTGGTAATTCTAGAGCT2461410.6445681812700413No Hit
CAGTGGCTGGTTGAGAT2332340.6107686862015546No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2310280.6049918452531481No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT2048900.5365443979687203No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT1987230.5203949065231978No Hit
TAACTGTGGTAATTCTAGAGCT1893630.4958839222634134No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1886690.494066548003126No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1865430.4884992026466835No Hit
TTGGTGACTCTAGATAACCTCGG1835800.480740009659318No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1822770.4773278502052049No Hit
AACTGTGGTAATTCTAGAGCTA1638150.42898150496971993No Hit
ATTGATCATCGACACTTCGAACG1632320.4274548058432825No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1555310.40728823642185097No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1545360.404682634996799No Hit
TCGCTTCTGGCGCCAAGCGC1511740.39587858274451326No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1472000.3854718892137031No Hit
ACTGTGGTAATTCTAGAGC1411000.36949785032645044No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG1397110.365860483110976No Hit
TTGGTGACTCTAGATAACC1223130.32030042924861185No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1158480.3033705667230236No Hit
TTTGGTGACTCTAGATAACCTC1112360.2912931458463008No Hit
AACTGTGGTAATTCTAGAGC1068320.27976041350868425No Hit
TTTGGTGACTCTAGATAACCTCG1062840.2783253687037311No Hit
ATTGATCATCGACACTTCGAACGCA966390.25306805639757507No Hit
TAGGAATAATGGAAT943450.24706077029800827No Hit
TGGTGACTCTAGATAACCT925350.24232093252982345No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC924630.2421323864970559No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG884120.2315240534589804No Hit
CACTCGCCGAATCCCGGGGCCGAGGG877090.229683110944597No Hit
TGGTGACTCTAGATAACCTC840780.22017462976433236No Hit
TTTCTAGAGCT824340.2158694953494728No Hit
ATCGGGGATT816170.2137300216165408No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA805510.2109384928536209No Hit
ACCGGCTCCGGGACGGCTGGG800080.20951654152316546No Hit
AGGAATAATGGAAT799220.2092913337618042No Hit
GGTTGGCCTCGGATAGCCGGTC792890.2076336998903893No Hit
AGCAGTGGCTGGTTGAGATTT769240.20144048645295443No Hit
TTGATCATCGACACTTCGAACG753730.197378890663753No Hit
TTTGGTGACTCTAGATAACCT751320.19674778519296154No Hit
ACGATGCCGACCGGCGATGCGGCGGCG733690.19213102608505558No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT727330.19046553612894201No Hit
TGGTGACTCTAGATAACCTCG718230.18808252377035187No Hit
AGCTAGGAATAATGGAAT704950.18460489694374999No Hit
ATGGGGATCGGGGATT696070.18227949587294995No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA692040.18122416182843143No Hit
AGGGCGCACCACCGGCCCGTCTCG686780.17984672831126836No Hit
AACGATGCCGACCGGCGATGCGGCGGC677550.17742967291898407No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC663320.17370327007692493No Hit
CGAGGGCGCACCACCGGCCCGTCTCG637810.1670229793881739No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG630110.1650065843155207No Hit
CGCTTCTGGCGCCAAGCGCCCGG619610.1622569546709936No Hit
GTCCGGTGCGGAGTGCCCTT589820.1544558625652353No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG588880.15420970524467761No Hit
TTGATCATCGACACTTCGAACGCA550690.14420890942329767No Hit
TTGGTGACTCTAGATAA548400.1436092282913008No Hit
AGAGCAGTGGCTGGTTGAGATTTAA529530.1386677510158507No Hit
TTGGTGACTCTAGATA515090.134886355580901No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA492480.12896548641301936No Hit
TCGCTTCTGGCGCCAAGCGCCCGG491680.12875599082105538No Hit
TTCGTGATGGGGATCGGGGATT490170.12836056789122338No Hit
TACCCTACGCCGGCAGGCGCGGGTA488740.12798609452058782No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG479130.1254695287221206No Hit
TCGTGATGGGGATCGGGGATT469830.12303414246553948No Hit
GTCCGGTGCGGAGTGCCCT469450.12293463205935659No Hit
ACTCGCCGAATCCCGGGGCCGAGGG467630.12245802958763857No Hit
TACCCTACGCCGGCAGGCGCGGGT457900.1199100394503768No Hit
TCACTGACCCGGTGAGGCGGGG453950.11887565496505469No Hit
TAACTGTGGTAATTCTAGAGCTA452680.1185430807128119No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG441040.11549491984973614No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG429340.1124310468172631No Hit
AACCCCATTCGTGATGGGGATCGGGGATT424830.11125001541756623No Hit
ACAAGCATCAAGCACGCAGC419150.10976259671462205No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT411900.10786404291244858No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT402840.10549150533345662No Hit
ACCGGCCCGTCTCG402240.10533438363948364No Hit
CCGGGGTCCGGTGCGGAGTGCCCT397540.10410359703669533No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC383760.10049503546511597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)