FastQCFastQC Report
Thu 22 Jun 2023
DRR277071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41915530
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATT32881427.844686682954981No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT18650614.449570362106837No Hit
TTGGTGACTCTAGATAACCTCG18499254.41345964133103No Hit
AACTGTGGTAATTCTAGAGCT15002503.5792223073405007No Hit
TTGGTGACTCTAGATAACCT14438553.4446779033928476No Hit
TTGGTGACTCTAGATAACCTC13676333.262831222699558No Hit
ACTGTGGTAATTCTAGAGCT10287102.4542454789430077No Hit
GAGGGCGCACCACCGGCCCGTCTCG4319541.0305345059456483No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT3767180.8987551869199794No Hit
AACGATGCCGACCGGCGATGCGGCGGCG3327410.793837033672245No Hit
ATTGATCATCGACACTTCGAACG3142010.7496052179228081No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG3106180.7410570735953953No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2694010.642723591947901No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT2690770.6419506087600467No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG2440920.5823426305238179No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2429620.5796467323686472No Hit
CAGTGGCTGGTTGAGAT2416230.5764522123422989No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2154170.5139312326481379No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG2132670.5088018689015742No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA2063780.4923664331573524No Hit
TAACTGTGGTAATTCTAGAGCT2033370.4851113656441896No Hit
ATTGATCATCGACACTTCGAACGCA1965590.46894074821432535No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1914410.45673047674692413No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1892170.4514245674574555No Hit
CTGTGGTAATTCTAGAGCT1870150.4461711446807425No Hit
TTCTAGAGCT1813080.43255566612184077No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1675830.39981123941412644No Hit
TTGGTGACTCTAGATAACCTCGG1540930.36762746409266445No Hit
AACGATGCCGACCGGCGATGCGGCGGC1512870.360933047965754No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1512270.36078990293096613No Hit
TTTGGTGACTCTAGATAACCTCG1476370.3522250583494948No Hit
AACTGTGGTAATTCTAGAGCTA1473990.3516572497115031No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT1369940.326833514928715No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1364750.3255953103778003No Hit
ACTGTGGTAATTCTAGAGC1319470.31479263175247935No Hit
GGTTGGCCTCGGATAGCCGGTC1164100.2777252249941728No Hit
AACTGTGGTAATTCTAGAGC1131520.2699524496051941No Hit
AGAGCAGTGGCTGGTTGAGATTTAA1061940.2533523970709663No Hit
TTTGGTGACTCTAGATAACCTC1059830.2528490036986291No Hit
TTGATCATCGACACTTCGAACG1055980.25193048972540727No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG1027580.2451549580787837No Hit
CACTCGCCGAATCCCGGGGCCGAGGG980750.23398248811359418No Hit
AGCAGTGGCTGGTTGAGATTT940240.2243178125148364No Hit
TGGTGACTCTAGATAACCTCG895490.21364157867024466No Hit
ATCGGGGATT883150.21069756245477514No Hit
GTTGGCCTCGGATAGCCGGTC858300.2047689722639795No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA851610.2031729051260953No Hit
TTGGTGACTCTAGATAACC849470.2026623545020187No Hit
AGGGCGCACCACCGGCCCGTCTCG834700.1991386008956585No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG813250.19402116590199386No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG808380.1928593053696327No Hit
ACGATGCCGACCGGCGATGCGGCGGC790160.1885124678132425No Hit
TTGATCATCGACACTTCGAACGCA764590.18241210358070145No Hit
TTTGGTGACTCTAGATAACCT743520.17738532710906912No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA742170.17706325078079652No Hit
TGGTGACTCTAGATAACCT733130.17490653225666No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG709820.16934534765515313No Hit
ATTGATCATCGACACTTCGAACGC688270.1642040551556905No Hit
TGGTGACTCTAGATAACCTC686890.1638748215756785No Hit
ATGGGGATCGGGGATT659870.15742852350906694No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG655670.15642650826555218No Hit
TTTCTAGAGCT641190.15297194142600606No Hit
AACGATGCCGACCGGCGATGCGGCGG620850.1481193247466989No Hit
ACAAGCATCAAGCACGCAGC610830.14572880266574229No Hit
AACCCCATTCGTGATGGGGATCGGGGATT582390.1389437280167995No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC574820.13713771482789314No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC572710.13663432145555596No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG561920.13406009657995496No Hit
ATTGATCATCGACACTTCGAAC555750.1325880884722202No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGG537790.12830328043090472No Hit
CGAGGGCGCACCACCGGCCCGTCTCG528600.12611077564807124No Hit
TAGGAATAATGGAAT526830.12568849779544716No Hit
GAACCCCATTCGTGATGGGGATCGGGGATT523990.12501094463078483No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC518980.12381568359030651No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT511510.12203352790719811No Hit
AGCTAGGAATAATGGAAT507830.12115557169383281No Hit
TTCGTGATGGGGATCGGGGATT492130.11740994328355146No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT489570.11679919113512342No Hit
TCACTGACCCGGTGAGGCGGGG482780.11517926649144124No Hit
ACTCGCCGAATCCCGGGGCCGAGGG482660.11515063748448366No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT479270.11434186803793248No Hit
CCGGGGTCCGGTGCGGAGTGCCCT477190.1138456319173347No Hit
TAACTGTGGTAATTCTAGAGCTA472310.11268138563439373No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC468890.11186545893610315No Hit
AGGAATAATGGAAT456760.10897154348280935No Hit
TCGTGATGGGGATCGGGGATT455960.10878068343642558No Hit
ACCCCATTCGTGATGGGGATCGGGGATT443270.10575316595066316No Hit
AAACGATGCCGACCGGCGATGCGGCGGC439030.10474160770482921No Hit
CGCTTCTGGCGCCAAGCGCCCGG438620.10464379193105752No Hit
TCGCTTCTGGCGCCAAGCGCCCGG425580.1015327731750022No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT424920.1013753136367356No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC423200.10096496453701051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)