FastQCFastQC Report
Thu 22 Jun 2023
DRR277072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35293218
Total Bases812.5 Mbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCG18761785.315973170822791No Hit
AACTGTGGTAATTCTAGAGCT17388554.926881419540718No Hit
TTGGTGACTCTAGATAACCT12690403.5957049878534737No Hit
TTGGTGACTCTAGATAACCTC12562143.5593637281814314No Hit
ACTGTGGTAATTCTAGAGCT11358713.218383203254518No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT10161872.879269892589562No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT6790151.9239248741783759No Hit
GAGGGCGCACCACCGGCCCGTCTCG4496451.2740266416057613No Hit
TTCTAGAGCT4248291.2037128493071956No Hit
GTTGGCCTCGGATAGCCGGTC3550571.0060204767952867No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG3115780.882826836589398No Hit
AACGATGCCGACCGGCGATGCGGCGGCG2752790.7799770482816274No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2554330.7237452816005613No Hit
TAACTGTGGTAATTCTAGAGCT2442990.6921981441306939No Hit
CTGTGGTAATTCTAGAGCT2181190.6180195866525972No Hit
ATTGATCATCGACACTTCGAACG1754720.49718333986999996No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1736360.49198120726763994No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1684490.4772843326443058No Hit
TTGGTGACTCTAGATAACCTCGG1523700.43172600469585964No Hit
TTTGGTGACTCTAGATAACCTCG1489330.42198758979699724No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1448630.4104556291806545No Hit
CAGTGGCTGGTTGAGAT1440320.4081010691629196No Hit
AACTGTGGTAATTCTAGAGCTA1429150.40493615515592823No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG1381750.39150581281650204No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1298860.36801971415584717No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1234290.349724414475325No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT1229410.3483417125635866No Hit
ACCGGCTCCGGGACGGCTGGG1182150.33495103790195613No Hit
ACTGTGGTAATTCTAGAGC1159350.3284908732323587No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1105640.3132726519865658No Hit
AACTGTGGTAATTCTAGAGC1083080.30688048905033255No Hit
GTCCGGTGCGGAGTGCCCTT1081120.3063251415611917No Hit
TCGCTTCTGGCGCCAAGCGC1039810.29462034320588165No Hit
ATTGATCATCGACACTTCGAACGCA1029930.29182093851572277No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT1003770.2844087495790268No Hit
AACGATGCCGACCGGCGATGCGGCGGC966630.2738854813409194No Hit
TTTGGTGACTCTAGATAACCTC955980.2708679044228837No Hit
GGTTGGCCTCGGATAGCCGGTC925670.26227985218009875No Hit
TTTCTAGAGCT921550.26111248909068024No Hit
AGGGCGCACCACCGGCCCGTCTCG915400.25936994467322305No Hit
TTGGTGACTCTAGATAACC883270.250266212619093No Hit
GTCCGGTGCGGAGTGCCCT839520.23787006330791374No Hit
TGGTGACTCTAGATAACCTCG836380.23698037396306565No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG816390.23131639625494052No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC791880.2243717192351233No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG789760.22377103725707304No Hit
TTGATCATCGACACTTCGAACG767870.21756871249314813No Hit
TGGTGACTCTAGATAACCT681140.19299458609866632No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC679080.1924109045539571No Hit
AGCTAGGAATAATGGAAT673740.190897865986604No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG659830.1869565988570382No Hit
CACTCGCCGAATCCCGGGGCCGAGGG654900.1855597299175156No Hit
ATCGGGGATT649870.18413452692242457No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG646340.18313433476085972No Hit
CGAGGGCGCACCACCGGCCCGTCTCG645190.182808493121823No Hit
TTTGGTGACTCTAGATAACCT613550.17384359793997814No Hit
TGGTGACTCTAGATAACCTC611330.17321458190635947No Hit
TACCCTACGCCGGCAGGCGCGGGTA591840.16769227447607638No Hit
CGGCCGAGGTGGGATCCCG577750.16370000604648746No Hit
TAGGAATAATGGAAT575540.1630738234184256No Hit
TACCCTACGCCGGCAGGCGCGGGT559660.15857437539416214No Hit
ACGATGCCGACCGGCGATGCGGCGGC556030.15754584917702885No Hit
ACCGGCCCGTCTCG526130.14907396656207433No Hit
TTGATCATCGACACTTCGAACGCA524360.1485724537785135No Hit
CCGGGGTCCGGTGCGGAGTGCCCT512710.14527153630479375No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA503590.1426874704369548No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG492720.13960755859666862No Hit
GAGGGCGCACCACCGGCCCGTCTC491640.1393015507965298No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA484050.1371509959788875No Hit
TCACTGACCCGGTGAGGCGGGG470320.13326073014934484No Hit
AGGAATAATGGAAT467790.13254387854346406No Hit
ACCCTACGCCGGCAGGCGCGGGT467130.13235687377671257No Hit
ACAAGCATCAAGCACGCAGC456510.12934779707534744No Hit
TCGCTTCTGGCGCCAAGCGCCCGG454470.12876978234175188No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT452900.12832493766932784No Hit
TAGATAACCTCG447010.12665606179634853No Hit
AGCAGTGGCTGGTTGAGATTT431840.12235778556662076No Hit
ATGGGGATCGGGGATT430690.12203194392758404No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG426480.12083908018815401No Hit
CGCTTCTGGCGCCAAGCGCCCGG415950.11785550413680045No Hit
AGATAACCTCG415840.1178243366756752No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC412800.11696298138639553No Hit
TAACTGTGGTAATTCTAGAGCTA410840.11640763389725471No Hit
AGGGCGCACCACCGGCCCGTCTCGC391770.11100432950035898No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT374640.10615070578149036No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC370390.10494650841983295No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC364860.10337963514689989No Hit
ATTGATCATCGACACTTCGAACGC363210.10291212323002115No Hit
ATTCTAGAGCT361940.10255228072430234No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC359940.10198559961293414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)