FastQCFastQC Report
Thu 22 Jun 2023
DRR277075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52748897
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATT37061737.026067293881046No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT30011725.689544560524175No Hit
AACTGTGGTAATTCTAGAGCT20692203.922773968145723No Hit
TTGGTGACTCTAGATAACCT17607383.337961739749743No Hit
ACTGTGGTAATTCTAGAGCT15854183.0055946003951513No Hit
TTGGTGACTCTAGATAACCTC14157512.683944272806311No Hit
TTGGTGACTCTAGATAACCTCG13454762.5507187382515317No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT5903021.1190793240662456No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG5872391.1132725675761523No Hit
GAGGGCGCACCACCGGCCCGTCTCG5730371.0863487818522537No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT5413871.0263475272288631No Hit
GAGGGCGCACCACCGGCCCGTCTCGC3684640.6985245587220525No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA3424860.6492761355749297No Hit
CTGTGGTAATTCTAGAGCT3156540.598408721228806No Hit
CAGTGGCTGGTTGAGAT3098220.5873525658745055No Hit
TTCTAGAGCT2795660.5299940205384769No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2718830.5154287870701827No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG2519120.47756827976895894No Hit
TAACTGTGGTAATTCTAGAGCT2431940.46104091996463925No Hit
ATTGATCATCGACACTTCGAACG2369240.4491544154942235No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA2267420.42985164220590244No Hit
GTTGGCCTCGGATAGCCGGTC2226730.4221377368326773No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA2086320.3955191707610493No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG2065420.39155700260424403No Hit
CACTCGCCGAATCCCGGGGCCGAGGG1970690.3735983332504564No Hit
ACTGTGGTAATTCTAGAGC1953700.37037741282059417No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT1912060.3624834088947869No Hit
AACTGTGGTAATTCTAGAGCTA1760670.3337832827101579No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA1729190.3278153854098598No Hit
GGTTGGCCTCGGATAGCCGGTC1628810.30878560361176843No Hit
TTGGTGACTCTAGATAACCTCGG1580900.2997029492389196No Hit
AACTGTGGTAATTCTAGAGC1571990.2980138143931237No Hit
ATTGATCATCGACACTTCGAACGCA1506070.28551687061816666No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1456730.27616312052932596No Hit
AGGGCGCACCACCGGCCCGTCTCG1432530.27157534687407775No Hit
AACCCCATTCGTGATGGGGATCGGGGATT1409230.26715819290022313No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1394800.26442259067521356No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1353100.2565172121039801No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1296270.2457435271110977No Hit
ATCGGGGATT1256350.2381755963541759No Hit
ACTCGCCGAATCCCGGGGCCGAGGG1170040.22181316890853658No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA1143560.21679315872709148No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG1134160.21501113094364796No Hit
TTGGTGACTCTAGATAACC1134050.21499027742703322No Hit
AGCAGTGGCTGGTTGAGATTT1106560.20977879404757982No Hit
TTGATCATCGACACTTCGAACG1096640.2078981860037756No Hit
AGAGCAGTGGCTGGTTGAGATTTAA1062380.20140326346539528No Hit
AACGATGCCGACCGGCGATGCGGCGGC1020900.1935395919273914No Hit
TTTCTAGAGCT1001450.18985231103505348No Hit
TGGTGACTCTAGATAACCT999550.18949211392988938No Hit
TTTGGTGACTCTAGATAACCTCG989060.18750344675453592No Hit
TTTGGTGACTCTAGATAACCTC948890.17988812164167148No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGG941920.1785667669979905No Hit
ATGGGGATCGGGGATT910640.17263678518244657No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC837730.15881469521533315No Hit
ACCGGCTCCGGGACGGCTGGG824450.15629710702765975No Hit
GAACCCCATTCGTGATGGGGATCGGGGATT815110.15452645388964245No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG808440.15326197247309264No Hit
TTTGGTGACTCTAGATAACCT806490.15289229649674002No Hit
TTGATCATCGACACTTCGAACGCA780170.14790261870309818No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCG758260.14374897734828465No Hit
GTTGAACCCCATTCGTGATGGGGATCGGG755470.1432200563359647No Hit
GTCCGGTGCGGAGTGCCCT745380.14130722013011948No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGA743140.1408825667008734No Hit
TTGAACCCCATTCGTGATGGGGATCGGGG739480.14018871332987304No Hit
ACGATGCCGACCGGCGATGCGGCGGCG739160.14012804855426644No Hit
TGGTGACTCTAGATAACCTC730660.13851664045221646No Hit
TCACTGACCCGGTGAGGCGGGG724110.13727490832651915No Hit
AGGGCGCACCACCGGCCCGTCTCGC710090.1346170328452555No Hit
ACAAGCATCAAGCACGCAGC705860.13381512034270593No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT696630.13206532072130342No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG690900.13097904208309796No Hit
CGAGGGCGCACCACCGGCCCGTCTCG683610.1295970226638104No Hit
TGGTGACTCTAGATAACCTCG681790.12925199175254792No Hit
ATTGATCATCGACACTTCGAACGC676330.12821689901876052No Hit
TCGCTTCTGGCGCCAAGCGC669190.12686331621303854No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG659190.12496754197533268No Hit
GTCCGGTGCGGAGTGCCCTT653290.12384903517508622No Hit
TAGGAATAATGGAAT651610.12353054510315163No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTG642260.12175799619089667No Hit
GAGGGCGCACCACCGGCCCGTCTC606140.11491045964430308No Hit
TTGAACCCCATTCGTGATGGGGATCGGG596720.11312464031238416No Hit
GTTGAACCCCATTCGTGATGGGGATCGG591810.11219381516167058No Hit
CCGGGGTCCGGTGCGGAGTGCCCT581760.11028856205277618No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC566490.10739371479179935No Hit
ACCCCATTCGTGATGGGGATCGGGGATT565470.10720034581955333No Hit
ATTGATCATCGACACTTCGAAC564780.10706953739715165No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT549400.10415383661956001No Hit
AGGAATAATGGAAT545950.10349979450755149No Hit
TTCGTGATGGGGATCGGGGATT544890.10329884243835469No Hit
TTGGCCTCGGATAGCCGGTC538460.1020798596035098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)