FastQCFastQC Report
Thu 22 Jun 2023
DRR277076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41284082
Total Bases958.2 Mbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTGTGGTAATTCTAGAGCT19147094.637886825241748No Hit
TTGGTGACTCTAGATAACCT18134674.392654292276621No Hit
TTGGTGACTCTAGATAACCTCG15673873.7965892035579234No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT15440313.7400153405373047No Hit
TTGGTGACTCTAGATAACCTC15046123.6445330188037124No Hit
ACTGTGGTAATTCTAGAGCT14869143.601664195900008No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT13131413.180744094055428No Hit
TTCTAGAGCT6022101.4586978099694696No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG5643811.367066851577322No Hit
GTTGGCCTCGGATAGCCGGTC4836391.1714902610647853No Hit
GAGGGCGCACCACCGGCCCGTCTCG4434001.0740217016330895No Hit
CTGTGGTAATTCTAGAGCT3100390.750989206929683No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2401980.5818174665964475No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT2249660.5449218902336257No Hit
TAACTGTGGTAATTCTAGAGCT2188860.530194664374516No Hit
TCGCTTCTGGCGCCAAGCGC2147560.5201908086511406No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT2106850.5103298651523849No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC2081550.5042015951814067No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA1904590.4613376167598931No Hit
ACTGTGGTAATTCTAGAGC1875250.45423076138643464No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1722890.4173254960592317No Hit
ATTGATCATCGACACTTCGAACG1694060.41034217498163095No Hit
TTGGTGACTCTAGATAACCTCGG1615180.39123553722231247No Hit
AACTGTGGTAATTCTAGAGCTA1592390.3857152497662416No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG1561930.37833710339011534No Hit
CAGTGGCTGGTTGAGAT1542870.37372031186257215No Hit
AACTGTGGTAATTCTAGAGC1492960.3616309065561879No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC1481870.35894464118155756No Hit
ACCGGCTCCGGGACGGCTGGG1386380.3358146609630317No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1367570.3312584254628697No Hit
TTGGTGACTCTAGATAACC1330650.322315511339213No Hit
ATTGATCATCGACACTTCGAACGCA1292020.3129583939882689No Hit
GGTTGGCCTCGGATAGCCGGTC1197090.28996405927107693No Hit
ATCGGGGATT1160290.28105021204056324No Hit
TTTCTAGAGCT1147400.2779279432687882No Hit
TTTGGTGACTCTAGATAACCTCG1116880.2705352634460904No Hit
GTCCGGTGCGGAGTGCCCTT1098090.26598387242811894No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1072170.25970542350923537No Hit
CACTCGCCGAATCCCGGGGCCGAGGG1030740.2496700786516217No Hit
TGGTGACTCTAGATAACCT1016650.24625714094841686No Hit
TAGGAATAATGGAAT1009930.24462939493241No Hit
TTTGGTGACTCTAGATAACCTC1009380.24449617167217136No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC991780.2402330273445344No Hit
TTGATCATCGACACTTCGAACG866420.2098678129745019No Hit
AGGGCGCACCACCGGCCCGTCTCG853290.2066874104164409No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG833850.20197857372727823No Hit
GTCCGGTGCGGAGTGCCCT817990.19813689935021445No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA813210.19697906810668578No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC802670.19442602599229408No Hit
TGGTGACTCTAGATAACCTC764530.18518759845501712No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT759760.18403218945258368No Hit
TTGATCATCGACACTTCGAACGCA759000.1838480991293448No Hit
TTTGGTGACTCTAGATAACCT757340.1834460071075336No Hit
AGGAATAATGGAAT750090.1816898823134786No Hit
TGGTGACTCTAGATAACCTCG734220.17784578569531956No Hit
CGAGGGCGCACCACCGGCCCGTCTCG682710.16536882181369567No Hit
AACGATGCCGACCGGCGATGCGGCGGC679770.16465668293169264No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG633070.15334481701688316No Hit
ATGGGGATCGGGGATT625300.15146273568587526No Hit
AGCTAGGAATAATGGAAT603700.14623069492013896No Hit
GAGGGCGCACCACCGGCCCGTCTC591450.1432634495784598No Hit
ATTCTAGAGCT583730.14139347945292813No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA580160.14052873938192448No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC574930.13926190728910964No Hit
TACCCTACGCCGGCAGGCGCGGGT564070.13663135345967No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG534250.12940823051363962No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG533920.12932829655749642No Hit
TCACTGACCCGGTGAGGCGGGG512620.12416892302461757No Hit
TACCCTACGCCGGCAGGCGCGGGTA501880.12156743608832091No Hit
ACTCGCCGAATCCCGGGGCCGAGGG498550.12076082980360325No Hit
ACGATGCCGACCGGCGATGCGGCGGCG485700.11764824999620918No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA479390.11611981586510753No Hit
CGGCCGAGGTGGGATCCCG469790.11379446441366918No Hit
AGCAGTGGCTGGTTGAGATTT467420.11322039327409533No Hit
TTGGTGACTCTAGATA462060.11192207204704224No Hit
CGCTTCTGGCGCCAAGCGCCCGG455180.11025557017351142No Hit
TGTGGTAATTCTAGAGCT443810.10750148204821412No Hit
ATTGATCATCGACACTTCGAACGC439700.10650594095806708No Hit
TAACTGTGGTAATTCTAGAGCTA432200.10468926013663088No Hit
TTCGTGATGGGGATCGGGGATT429860.10412245572034279No Hit
CCGGGGTCCGGTGCGGAGTGCCCT422530.10234695299752579No Hit
TCGCTTCTGGCGCCAAGCGCCCGG421160.10201510596747677No Hit
ACCGGCCCGTCTCG420430.10183828236752362No Hit
AACCCCATTCGTGATGGGGATCGGGGATT417020.10101229815404397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)