FastQCFastQC Report
Thu 22 Jun 2023
DRR277079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR277079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57490004
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length10-39
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATT43952347.645214288035186No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT25805094.488622056801388No Hit
AACTGTGGTAATTCTAGAGCT17953333.122861149913992No Hit
TTGGTGACTCTAGATAACCTC16619182.890794719722058No Hit
TTGGTGACTCTAGATAACCT16267952.829700620650505No Hit
TTGGTGACTCTAGATAACCTCG14952472.6008817115406706No Hit
ACTGTGGTAATTCTAGAGCT12832622.232148044380028No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT5361170.9325395072158978No Hit
GAGGGCGCACCACCGGCCCGTCTCG5266610.916091430433715No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG4160330.7236614559985072No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT3883400.6754913428080471No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC3798440.6607131215367458No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA3520070.6122925300196534No Hit
AACGATGCCGACCGGCGATGCGGCGGCG3517940.6119220308281766No Hit
CAGTGGCTGGTTGAGAT3423630.5955174398665897No Hit
GAGGGCGCACCACCGGCCCGTCTCGC3420120.5949068989454237No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3411830.5934649091344645No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG3224170.5608227127623787No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG3196350.55598361064647No Hit
ATTGATCATCGACACTTCGAACG3137010.5456618162698337No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA2754670.4791563416833298No Hit
TTCTAGAGCT2707920.4710244932319017No Hit
GTTGGCCTCGGATAGCCGGTC2705750.47064703630912946No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA2467450.4291963521171437No Hit
CTGTGGTAATTCTAGAGCT2367210.4117602774910226No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2357720.41010955574120334No Hit
TAACTGTGGTAATTCTAGAGCT2350880.4089197836897002No Hit
ATTGATCATCGACACTTCGAACGCA2119940.36874932205605687No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT2095830.36455554951779096No Hit
GGTTGGCCTCGGATAGCCGGTC1967870.3422977671039995No Hit
TTGGTGACTCTAGATAACCTCGG1809820.3148060313232888No Hit
AACTGTGGTAATTCTAGAGCTA1808210.3145259826386514No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1756830.30558877678978763No Hit
AGCAGTGGCTGGTTGAGATTT1606850.27950076329791174No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1570750.27322141080386775No Hit
ACTGTGGTAATTCTAGAGC1539000.2676987115881919No Hit
AACGATGCCGACCGGCGATGCGGCGGC1507400.26220210386487364No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG1440600.25058269260165644No Hit
ATCGGGGATT1412650.2457209778590379No Hit
CACTCGCCGAATCCCGGGGCCGAGGG1382470.24047136959670415No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1380120.24006260288310294No Hit
AACTGTGGTAATTCTAGAGC1373810.2389650207712631No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA1354740.23564792237620993No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG1337310.23261609096426572No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA1324420.23037396205434252No Hit
TTGATCATCGACACTTCGAACG1288240.22408069409770784No Hit
AGGGCGCACCACCGGCCCGTCTCG1258010.21882238867125492No Hit
TTGGTGACTCTAGATAACC1239780.21565140263340388No Hit
TTTGGTGACTCTAGATAACCTC1213410.21106451827695127No Hit
TAGGAATAATGGAAT1202480.2091633182004997No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG1198810.20852494635415228No Hit
AGAGCAGTGGCTGGTTGAGATTTAA1163120.2023169106058855No Hit
AGGAATAATGGAAT1159290.2016507078343567No Hit
TTTGGTGACTCTAGATAACCTCG1081850.18818054004657922No Hit
TTGATCATCGACACTTCGAACGCA1027360.1787023705895028No Hit
ATGGGGATCGGGGATT999450.1738476135781796No Hit
CCGGGGTCCGGTGCGGAGTGCCCT988610.17196206839714256No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT972490.1691581026851207No Hit
ACCGGCTCCGGGACGGCTGGG923280.16059835375902914No Hit
GTCCGGTGCGGAGTGCCCT921220.16024003059731914No Hit
AACCCCATTCGTGATGGGGATCGGGGATT910790.15842580216205934No Hit
TGGTGACTCTAGATAACCT888710.15458513448703187No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC883410.15366323509039936No Hit
CGAGGGCGCACCACCGGCCCGTCTCG879220.15293441273721253No Hit
TGGTGACTCTAGATAACCTC865190.15049398848537215No Hit
GAACCCCATTCGTGATGGGGATCGGGGATT849930.1478396139962001No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG831990.14471907151024027No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC831630.14465645192858223No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC823250.1431988072222086No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGG820420.1427065477330633No Hit
TTTGGTGACTCTAGATAACCT781340.13590884425751648No Hit
TGGTGACTCTAGATAACCTCG744420.12948685827191803No Hit
CGCTTCTGGCGCCAAGCGCCCGG728010.12663244900800494No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC724430.12600973205707205No Hit
CGGTTGGCCTCGGATAGCCGGTC723760.1258931900578751No Hit
TACCCTACGCCGGCAGGCGCGGGTA721010.12541484603132053No Hit
GTTGAACCCCATTCGTGATGGGGATCGGG710340.12355887120828868No Hit
TCACTGACCCGGTGAGGCGGGG706150.12283004885510183No Hit
ACAAGCATCAAGCACGCAGC702120.12212905742709637No Hit
AGCTAGGAATAATGGAAT696720.12118976370222552No Hit
ACGATGCCGACCGGCGATGCGGCGGC688110.11969211204090366No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT680900.11843798097491869No Hit
ACTCGCCGAATCCCGGGGCCGAGGG678690.11805356632085119No Hit
TTCGTGATGGGGATCGGGGATT676560.11768306712937435No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG676490.1176708910996075No Hit
ATTGATCATCGACACTTCGAACGC673600.11716819501351923No Hit
GTCCGGTGCGGAGTGCCCTT667040.11602712708108352No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC658700.11457644010600522No Hit
TCGTGATGGGGATCGGGGATT656320.11416245509393251No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT655480.11401634273673036No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC646960.11253434597082303No Hit
TCGCTTCTGGCGCCAAGCGCCCGG645370.11225777615183329No Hit
ACCCCATTCGTGATGGGGATCGGGGATT644880.11217254394346536No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT641150.11152373550017496No Hit
ATTGATCATCGACACTTCGAAC641120.11151851720170344No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG639800.11128891206895725No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCG633580.11020698485253193No Hit
TTTCTAGAGCT626530.10898068471172832No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC626360.10895111435372312No Hit
TCGCTTCTGGCGCCAAGCGC623910.10852495331188357No Hit
AGGGCGCACCACCGGCCCGTCTCGC623140.1083910169844483No Hit
GAGGGCGCACCACCGGCCCGTCTC602890.10486866551618261No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATT602800.10485301062076809No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTG600610.10447207483234824No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA594710.10344580946628566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)